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Simple Colorimetric Detection of Hg2...

Kumah, Philip Selasi.

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Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs).

Record Type:	Electronic resources : Monograph/item
Title/Author:	Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs)./
Author:	Kumah, Philip Selasi.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	44 p.
Notes:	Source: Masters Abstracts International, Volume: 58-01.
Contained By:	Masters Abstracts International58-01(E).
Subject:	Chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10827582
ISBN:	9780438338128

Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs).
Kumah, Philip Selasi.

Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs). - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 44 p.

Source: Masters Abstracts International, Volume: 58-01.

Thesis (M.S.)--North Carolina Agricultural and Technical State University, 2018.

Hg2+ and other heavy metal ions (HMIs) are common toxic contaminants that have severe adverse effects like cancer, neurological, cardiovascular, pulmonary, gastrointestinal disorders, renal dysfunction, and oxidative DNA damage on humans at higher concentrations. Mercury pollution comes from multiple sources including nature and human activities. Water-soluble Hg2+ is one of the most common and stable forms of mercury pollution which makes environmental detection of aqueous Hg2+ a vital and critical aspect of research. This study developed a simple, cost-effective and sensitive colorimetric sensor for the detection of Hg2+ in simulated urine, human serum albumin (HSA), and water samples using L-cysteine functionalized gold nanoparticles (AuNPs). The presence of Hg2+ was determined by the colorimetric response of the gold nanoparticles and by measuring the UV-vis absorption spectra. It was observed in the study that, Hg2+ detection in HSA did not yield a colorimetric response due to the high affinity between HSA and Hg2+ which limits a metal to ligand binding between Hg2+ and L-cysteine gold nanoparticle thus preventing aggregation of AuNPs. On the contrary, the UV-vis absorption spectra showed an increase in absorption as the concentration of the Hg 2+ ion increases with a correlation coefficient (R2) of 0.9839. There was a noticeable color change from red to blue observed as Hg2+ was detected in tap water, DI water and simulated urine with a corresponding UV-vis adsorption spectrum showing a red shift from 520 nm to 523 nm, 522 nm, and 533 nm respectively. A second absorbance peak was also observed at longer wavelength indicating aggregation between Hg2+ and L-cysteine gold nanoparticles. This study has a potential to be used for point of care diagnostics.

ISBN: 9780438338128Subjects--Topical Terms:

516420
Chemistry.

Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs).
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245 1 0 $a Simple Colorimetric Detection of Hg2+ in Simulated Urine, HSA, and Water Samples Using L-Cysteine Gold Nanoparticles (AuNPs).
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