東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Combined Effects of Environmental St...

Serafin, Jennifer.

FindBook

Google Book

Amazon

博客來

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome./
作者:	Serafin, Jennifer.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
面頁冊數:	120 p.
附註:	Source: Masters Abstracts International, Volume: 56-06.
Contained By:	Masters Abstracts International56-06(E).
標題:	Aquatic sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10275318
ISBN:	9780355157703

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
Serafin, Jennifer.

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 120 p.

Source: Masters Abstracts International, Volume: 56-06.

Thesis (M.S.)--Purdue University, 2017.

This item is not available from ProQuest Dissertations & Theses.

Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe. Although the toxicity of PAHs to fish has been well studied, the combined effects of extreme abiotic factors and oil are poorly understood. The overarching hypothesis tested in this work was that exposure to oil in combination with suboptimal environmental conditions will increase the sensitivity of fish larvae to PAHs. Gulf of Mexico killifish Fundulus grandis larvae (< 24 h post hatch) were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 25, 30°C; and salinity 3, 10, 30 ppt) combined with varying concentrations of high energy water accommodated fractions (HEWAF) (SigmaPAHs 0--125 ppb) for a total of 48 h. Larvae survival and development were negatively affected by PAHs, starting with the lowest concentration tested (15 ppb). High temperature + hypoxia + PAH resulted in the lowest survival and salinity had little impact on endpoints tested. Expression (via qPCR) of the hepatic detoxifying gene cyp1a was highly induced in PAH-exposed larvae, but only under normoxic conditions. Since hypoxia + PAHs resulted in significant negative effects, we next used RNASeq to evaluate the transcriptome of fish larvae in order to better understand the molecular mechanisms behind these effects. A total of 380 and 110 differentially expressed genes were identified due to PAH and hypoxia exposure, respectively. These expression changes were primarily associated with toxicological effects on the hepatic and cardiac systems. Ugt1a1, a detoxiying enzyme, was the only gene found up-regulated across all comparisons in response to PAHs and was identified as a potential biomarker of exposure to oil. The expression of cyp1a was not induced, in contrast to results from qPCR, but other genes associated with the aryl-hydrocarbon receptor (AhR) were ( ahrr, por). This work highlights the need for more studies examining the combined impact of suboptimal water quality parameters in the presence of pollution in fish early life-stages.

ISBN: 9780355157703Subjects--Topical Terms:

3174300
Aquatic sciences.

Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
LDR:03188nmm a2200313 4500 001 2158718
005 20180618102543.5
008 190424s2017 ||||||||||||||||| ||eng d
020 $a 9780355157703
035 $a (MiAaPQ)AAI10275318
035 $a (MiAaPQ)purdue:21444
035 $a AAI10275318
040 $a MiAaPQ $c MiAaPQ
100 1 $a Serafin, Jennifer. $3 3346551
245 1 0 $a Combined Effects of Environmental Stressors and Crude Oil on Fundulus grandis Development and Transcriptome.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 120 p.
500 $a Source: Masters Abstracts International, Volume: 56-06.
500 $a Adviser: Maria Sepulveda.
502 $a Thesis (M.S.)--Purdue University, 2017.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe. Although the toxicity of PAHs to fish has been well studied, the combined effects of extreme abiotic factors and oil are poorly understood. The overarching hypothesis tested in this work was that exposure to oil in combination with suboptimal environmental conditions will increase the sensitivity of fish larvae to PAHs. Gulf of Mexico killifish Fundulus grandis larvae (< 24 h post hatch) were exposed to varying environmental conditions (dissolved oxygen (DO) 2, 6 ppm; temperature 20, 25, 30°C; and salinity 3, 10, 30 ppt) combined with varying concentrations of high energy water accommodated fractions (HEWAF) (SigmaPAHs 0--125 ppb) for a total of 48 h. Larvae survival and development were negatively affected by PAHs, starting with the lowest concentration tested (15 ppb). High temperature + hypoxia + PAH resulted in the lowest survival and salinity had little impact on endpoints tested. Expression (via qPCR) of the hepatic detoxifying gene cyp1a was highly induced in PAH-exposed larvae, but only under normoxic conditions. Since hypoxia + PAHs resulted in significant negative effects, we next used RNASeq to evaluate the transcriptome of fish larvae in order to better understand the molecular mechanisms behind these effects. A total of 380 and 110 differentially expressed genes were identified due to PAH and hypoxia exposure, respectively. These expression changes were primarily associated with toxicological effects on the hepatic and cardiac systems. Ugt1a1, a detoxiying enzyme, was the only gene found up-regulated across all comparisons in response to PAHs and was identified as a potential biomarker of exposure to oil. The expression of cyp1a was not induced, in contrast to results from qPCR, but other genes associated with the aryl-hydrocarbon receptor (AhR) were ( ahrr, por). This work highlights the need for more studies examining the combined impact of suboptimal water quality parameters in the presence of pollution in fish early life-stages.
590 $a School code: 0183.
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Toxicology. $3 556884
690 $a 0792
690 $a 0383
710 2 $a Purdue University. $b Forestry and Natural Resources. $3 2093037
773 0 $t Masters Abstracts International $g 56-06(E).
790 $a 0183
791 $a M.S.
792 $a 2017
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10275318