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Drosophila midline development: The ...

Moawad, Amanda Christine.

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Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis./
Author:	Moawad, Amanda Christine.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
Description:	73 p.
Notes:	Source: Masters Abstracts International, Volume: 55-05.
Contained By:	Masters Abstracts International55-05(E).
Subject:	Developmental biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10119781
ISBN:	9781339809724

Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis.
Moawad, Amanda Christine.

Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 73 p.

Source: Masters Abstracts International, Volume: 55-05.

Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2016.

The developing midline of Drosophila consists of diverse cell types that must migrate and differentiate appropriately to form a functional central nervous system. Despite the paucity of midline cells, much is unknown about how these cells migrate to their final locations and acquire fates. In a previous RNA-sequencing screen, we found that 18-Wheeler (18w), a Toll-family receptor, is present in the midline during embryonic development. Fluorescence in situ hybridizations and genetic analyses revealed that 18w is expressed posteriorly in segments in the median neuroblast. 18w overexpression led to midline disorganization, while heterozygous 18w loss of function mutants showed no phenotype, indicating that one wild-type copy of 18w is sufficient to maintain function.

ISBN: 9781339809724Subjects--Topical Terms:

592588
Developmental biology.

Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis.
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245 1 0 $a Drosophila midline development: The role of 18-Wheeler and an optimized protocol for transcriptome analysis.
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300 $a 73 p.
500 $a Source: Masters Abstracts International, Volume: 55-05.
500 $a Adviser: Stephen T. Crews.
502 $a Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2016.
520 $a The developing midline of Drosophila consists of diverse cell types that must migrate and differentiate appropriately to form a functional central nervous system. Despite the paucity of midline cells, much is unknown about how these cells migrate to their final locations and acquire fates. In a previous RNA-sequencing screen, we found that 18-Wheeler (18w), a Toll-family receptor, is present in the midline during embryonic development. Fluorescence in situ hybridizations and genetic analyses revealed that 18w is expressed posteriorly in segments in the median neuroblast. 18w overexpression led to midline disorganization, while heterozygous 18w loss of function mutants showed no phenotype, indicating that one wild-type copy of 18w is sufficient to maintain function.
520 $a To further analyze midline gene expression, a protocol was optimized to dissociate cells from tissue for fluorescence-activated cell sorting. Isolated midline cells would subsequently undergo single-cell RNA-sequencing, revealing expression differences among cell types and allowing for transcriptome analysis.
590 $a School code: 0153.
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650 4 $a Neurosciences. $3 588700
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