東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

DNA polymerase exchange and lesion b...

Kath, James Evon.

FindBook

Google Book

Amazon

博客來

DNA polymerase exchange and lesion bypass in Escherichia coli.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	DNA polymerase exchange and lesion bypass in Escherichia coli./
作者:	Kath, James Evon.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2016,
面頁冊數:	131 p.
附註:	Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.
Contained By:	Dissertation Abstracts International77-09B(E).
標題:	Biophysics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10102867
ISBN:	9781339664958

DNA polymerase exchange and lesion bypass in Escherichia coli.
Kath, James Evon.

DNA polymerase exchange and lesion bypass in Escherichia coli. - Ann Arbor : ProQuest Dissertations & Theses, 2016 - 131 p.

Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.

Thesis (Ph.D.)--Harvard University, 2016.

This item is not available from ProQuest Dissertations & Theses.

Translesion synthesis (TLS) alleviates replication stalling at DNA lesions. Bypass of lesions by specialized translesion DNA polymerases involves exchange with high-fidelity replicative polymerases. As a consequence of their lesion bypass activity, TLS polymerases are mutagenic, requiring careful regulation of polymerase selection. In this dissertation, I describe a single-molecule reconstitution of polymerase exchange and lesion bypass. Using Escherichia coli polymerases as a model system, I have determined that the dimeric processivity clamp can simultaneously bind a replicative polymerase and a translesion polymerase, facilitating rapid exchange during synthesis and lesion bypass. Overlapping sets of polymerase-clamp interactions additionally allow the TLS polymerase Polymerase IV to displace the replicative polymerase Polymerase III. I finally describe the observation of single Polymerase IV molecules in living cells and initial efforts to determine their localization and dynamics during TLS.

ISBN: 9781339664958Subjects--Topical Terms:

518360
Biophysics.

DNA polymerase exchange and lesion bypass in Escherichia coli.
LDR:02004nmm a2200313 4500 001 2123940
005 20171023101700.5
008 180830s2016 ||||||||||||||||| ||eng d
020 $a 9781339664958
035 $a (MiAaPQ)AAI10102867
035 $a AAI10102867
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kath, James Evon. $3 3285895
245 1 0 $a DNA polymerase exchange and lesion bypass in Escherichia coli.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2016
300 $a 131 p.
500 $a Source: Dissertation Abstracts International, Volume: 77-09(E), Section: B.
500 $a Adviser: James M. Hogle.
502 $a Thesis (Ph.D.)--Harvard University, 2016.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a Translesion synthesis (TLS) alleviates replication stalling at DNA lesions. Bypass of lesions by specialized translesion DNA polymerases involves exchange with high-fidelity replicative polymerases. As a consequence of their lesion bypass activity, TLS polymerases are mutagenic, requiring careful regulation of polymerase selection. In this dissertation, I describe a single-molecule reconstitution of polymerase exchange and lesion bypass. Using Escherichia coli polymerases as a model system, I have determined that the dimeric processivity clamp can simultaneously bind a replicative polymerase and a translesion polymerase, facilitating rapid exchange during synthesis and lesion bypass. Overlapping sets of polymerase-clamp interactions additionally allow the TLS polymerase Polymerase IV to displace the replicative polymerase Polymerase III. I finally describe the observation of single Polymerase IV molecules in living cells and initial efforts to determine their localization and dynamics during TLS.
590 $a School code: 0084.
650 4 $a Biophysics. $3 518360
650 4 $a Biochemistry. $3 518028
650 4 $a Microbiology. $3 536250
690 $a 0786
690 $a 0487
690 $a 0410
710 2 $a Harvard University. $b Biophysics. $3 3174713
773 0 $t Dissertation Abstracts International $g 77-09B(E).
790 $a 0084
791 $a Ph.D.
792 $a 2016
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10102867