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Cloning and characterization of a ph...

Shen, Wei-Chiang.

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Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea./
Author:	Shen, Wei-Chiang.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 1999,
Description:	86 p.
Notes:	Source: Dissertation Abstracts International, Volume: 60-06, Section: B, page: 2437.
Contained By:	Dissertation Abstracts International60-06B.
Subject:	Plant pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9934494
ISBN:	9780599351035

Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea.
Shen, Wei-Chiang.

Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea. - Ann Arbor : ProQuest Dissertations & Theses, 1999 - 86 p.

Source: Dissertation Abstracts International, Volume: 60-06, Section: B, page: 2437.

Thesis (Ph.D.)--Texas A&M University, 1999.

In heterothallic ascomycetes one mating partner serves as the source of female tissue and is fertilized with spermatia from a partner of the opposite mating type. The role of pheromone signaling in mating is thought to involve recognition of cells of the opposite mating type. I have isolated a putative pheromone precursor gene from Mat 1-1 strains of M. grisea. The gene, MF1-1, is present in both mating types of the fungus but it is expressed only in the Mat 1-1 strains. The MF1-1 gene is predicted to encode a 26 amino acid polypeptide that is processed to produce a lipopeptide pheromone. The mature pheromone is predicted to contain a C-terminal cysteine residue that is farnesylated and methyl esterified. Expression of this M. grisea putative pheromone precursor gene was observed in defined nutritional conditions and in field isolates. This suggests that the requirement for complex media for mating and the poor fertility of field isolates may not be due to limitation for pheromone precursor gene expression. Detection of putative pheromone precursor gene mRNA in conidia, suggests that pheromones may be important for the fertility of conidia acting as spermatia. Characterization of the MF1-1 pheromone gene disruption mutants indicates that pheromones play the roles in male fertility in M. grisea. Analysis of the N. crassa transformants of the M. grisea MF1-1 pheromone genomic clone suggests that mating type specific regulation of the pheromone precursor gene is conserved in these two fungal species.

ISBN: 9780599351035Subjects--Topical Terms:

3174872
Plant pathology.

Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea.
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100 1 $a Shen, Wei-Chiang. $3 2139006
245 1 0 $a Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 1999
300 $a 86 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-06, Section: B, page: 2437.
500 $a Chair: Daniel J. Ebbole.
502 $a Thesis (Ph.D.)--Texas A&M University, 1999.
520 $a In heterothallic ascomycetes one mating partner serves as the source of female tissue and is fertilized with spermatia from a partner of the opposite mating type. The role of pheromone signaling in mating is thought to involve recognition of cells of the opposite mating type. I have isolated a putative pheromone precursor gene from Mat 1-1 strains of M. grisea. The gene, MF1-1, is present in both mating types of the fungus but it is expressed only in the Mat 1-1 strains. The MF1-1 gene is predicted to encode a 26 amino acid polypeptide that is processed to produce a lipopeptide pheromone. The mature pheromone is predicted to contain a C-terminal cysteine residue that is farnesylated and methyl esterified. Expression of this M. grisea putative pheromone precursor gene was observed in defined nutritional conditions and in field isolates. This suggests that the requirement for complex media for mating and the poor fertility of field isolates may not be due to limitation for pheromone precursor gene expression. Detection of putative pheromone precursor gene mRNA in conidia, suggests that pheromones may be important for the fertility of conidia acting as spermatia. Characterization of the MF1-1 pheromone gene disruption mutants indicates that pheromones play the roles in male fertility in M. grisea. Analysis of the N. crassa transformants of the M. grisea MF1-1 pheromone genomic clone suggests that mating type specific regulation of the pheromone precursor gene is conserved in these two fungal species.
590 $a School code: 0803.
650 4 $a Plant pathology. $3 3174872
650 4 $a Microbiology. $3 536250
650 4 $a Genetics. $3 530508
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710 2 $a Texas A&M University. $3 718977
773 0 $t Dissertation Abstracts International $g 60-06B.
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