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Enzymatic signal amplification using...

Abel, Biebele.

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Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering./
Author:	Abel, Biebele.
Description:	197 p.
Notes:	Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
Contained By:	Dissertation Abstracts International76-11B(E).
Subject:	Environmental science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3707554
ISBN:	9781321822267

Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering.
Abel, Biebele.

Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering. - 197 p.

Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.

Thesis (Ph.D.)--Morgan State University, 2015.

In this work, we report the combined use of enzymes with plasmonic nanostructures to design a new hybrid system (enzyme-nanoparticle) for applications in bio- and nanotechnology. In this regard, current enzyme/nanoparticle hybrid systems have certain drawbacks, such as, cost effectiveness, as well as lengthy and complex preparation procedures. Subsequently, there is a need for the development of new hybrid systems, which can be prepared in a facile and inexpensive manner and can be applicable to environmental sensing. In addition, the fundamental study of the interactions of plasmonic nanostructures with enzymes (i.e., hybrid systems) can lead to the improvement of the stability and increase the efficiency of enzymes used in bioenvironmental science and engineering. In this dissertation, several fundamental issues were investigated, which include: the comparison of enzyme immobilization methods and the effect of nanoparticle loading, the application of biologically relevant enzymes to the hybrid system, the use of variety of plasmonic nanostructures in the hybrid system for enhanced enzyme activity, and the potential bioenvironmental applications. It was observed from the results that the hybrid systems show good sensitivity and acceptable reproducibility. This can be employed as a screening tool for pesticides detection.

ISBN: 9781321822267Subjects--Topical Terms:

677245
Environmental science.

Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering.
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100 1 $a Abel, Biebele. $3 3277041
245 1 0 $a Enzymatic signal amplification using plasmonic nanostructures for applications in bioenvironmental science and engineering.
300 $a 197 p.
500 $a Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
500 $a Adviser: Kadir Aslan.
502 $a Thesis (Ph.D.)--Morgan State University, 2015.
520 $a In this work, we report the combined use of enzymes with plasmonic nanostructures to design a new hybrid system (enzyme-nanoparticle) for applications in bio- and nanotechnology. In this regard, current enzyme/nanoparticle hybrid systems have certain drawbacks, such as, cost effectiveness, as well as lengthy and complex preparation procedures. Subsequently, there is a need for the development of new hybrid systems, which can be prepared in a facile and inexpensive manner and can be applicable to environmental sensing. In addition, the fundamental study of the interactions of plasmonic nanostructures with enzymes (i.e., hybrid systems) can lead to the improvement of the stability and increase the efficiency of enzymes used in bioenvironmental science and engineering. In this dissertation, several fundamental issues were investigated, which include: the comparison of enzyme immobilization methods and the effect of nanoparticle loading, the application of biologically relevant enzymes to the hybrid system, the use of variety of plasmonic nanostructures in the hybrid system for enhanced enzyme activity, and the potential bioenvironmental applications. It was observed from the results that the hybrid systems show good sensitivity and acceptable reproducibility. This can be employed as a screening tool for pesticides detection.
590 $a School code: 0755.
650 4 $a Environmental science. $3 677245
650 4 $a Nanotechnology. $3 526235
650 4 $a Analytical chemistry. $3 3168300
690 $a 0768
690 $a 0652
690 $a 0486
710 2 $a Morgan State University. $b Biology. $3 3277042
773 0 $t Dissertation Abstracts International $g 76-11B(E).
790 $a 0755
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3707554