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Chemical Tools to Study Protein Pyro...

Conway, John Henry.

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Chemical Tools to Study Protein Pyrophosphorylation.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Chemical Tools to Study Protein Pyrophosphorylation./
作者:	Conway, John Henry.
面頁冊數:	160 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
Contained By:	Dissertation Abstracts International76-11B(E).
標題:	Analytical chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3712212
ISBN:	9781321888843

Chemical Tools to Study Protein Pyrophosphorylation.
Conway, John Henry.

Chemical Tools to Study Protein Pyrophosphorylation. - 160 p.

Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.

Thesis (Ph.D.)--Princeton University, 2015.

This thesis describes the development of tools to study protein pyrophosphorylation, a recently discovered post-translational modification (PTM). Protein pyrophosphorylation occurs when an inositol pyrophosphate (PP-IP) transfer its beta-phosphoryl group to a phosphorylated serine residue on a protein. Genetic studies in mice and mammals have suggested a role for pyrophosphorylation in numerous biological processes, including signaling and body weight regulation. Detection of this modification in complex samples, however, has been elusive due to a variety of technical challenges. Herein, the development of chemical methods to synthesize model pyrophosphorylated peptides as well an affinity reagent that can selectively enrich for these pyrophosphorylated peptides is described.

ISBN: 9781321888843Subjects--Topical Terms:

3168300
Analytical chemistry.

Chemical Tools to Study Protein Pyrophosphorylation.
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245 1 0 $a Chemical Tools to Study Protein Pyrophosphorylation.
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500 $a Source: Dissertation Abstracts International, Volume: 76-11(E), Section: B.
500 $a Adviser: Dorothea Fiedler.
502 $a Thesis (Ph.D.)--Princeton University, 2015.
520 $a This thesis describes the development of tools to study protein pyrophosphorylation, a recently discovered post-translational modification (PTM). Protein pyrophosphorylation occurs when an inositol pyrophosphate (PP-IP) transfer its beta-phosphoryl group to a phosphorylated serine residue on a protein. Genetic studies in mice and mammals have suggested a role for pyrophosphorylation in numerous biological processes, including signaling and body weight regulation. Detection of this modification in complex samples, however, has been elusive due to a variety of technical challenges. Herein, the development of chemical methods to synthesize model pyrophosphorylated peptides as well an affinity reagent that can selectively enrich for these pyrophosphorylated peptides is described.
520 $a First, to facilitate the study of protein pyrophosphorylation, access to model pyrophosphopeptides was needed. Three solution phase methods to pyrophosphorylate phosphoserine, which utilize electrophilic phosphorus(III) and phosphorus(V) reagents, were explored. The optimized pyrophosphorylation methodology proved to be robust, highly selective, and compatible with complex substrates as well as aqueous solvent. Second, an affinity chromatography reagent was designed to enable enrichment of natively pyrophosphorylated species. The initial design of the reagent was based on existing zinc(II)-dipicolylamine (Zn-dpa) complexes that had been shown to bind inorganic pyrophosphate (PPi) with high specificity in aqueous solution. The affinity of these complexes toward diphosphate esters and other biologically relevant anions was characterized using isothermal titration calorimetry (ITC). Once a complex with ideal affinity and selectivity toward diphosphate esters was identified, a solid-phase analog was synthesized, which generated a pyrophosphopeptide affinity reagent. The reagent was shown to be easy to synthesize, recyclable, and compatible with chemically mild conditions. This affinity reagent, in conjunction with mass spectrometry (MS) methods developed by our group will ultimately enable the annotation of the pyrophosphoproteome.
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