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Opene, Belita.

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PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi .

Record Type:	Electronic resources : Monograph/item
Title/Author:	PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi ./
Author:	Opene, Belita.
Description:	33 p.
Notes:	Source: Masters Abstracts International, Volume: 54-06.
Contained By:	Masters Abstracts International54-06(E).
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1592289
ISBN:	9781321867961

PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi .
Opene, Belita.

PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi . - 33 p.

Source: Masters Abstracts International, Volume: 54-06.

Thesis (M.S.)--Adelphi University, 2015.

Estuaries are subject to a diverse array of disturbances such as the daily and seasonal tidal cycles and the variation of the flow of freshwater into the system. These disturbances allow for the accumulation of sediments and the formation of salt marsh environments within the estuaries. Due to the physical, biological and chemical properties of these sediments, estuaries contain diverse microbial communities that exist as single cells or micro-colonies. Scientists have been successful in culturing and identifying some of the resident microbial phyla present in estuary sediments. However, there are still many more organisms that are not culturable and can only be identified using molecular techniques. The phylum Chloroflexi has a few cultured representatives that have mainly been isolated from geothermal hot springs. However, molecular evidence indicates that this phylum is abundant in diverse environmental samples, including salt marsh sediments. In this study, metagenomic DNA was extracted from sediment samples from two salt marshes; one in New Hampshire and the other in Long Island, NY, and this DNA was subjected to polymerase chain reaction using primers targeting the Chloroflexi 16S rDNA gene. The 16S primers produced amplification product for one of the two samples indicating the presence of Chloroflexi organisms. These findings will pave the way for future qPCR work utilizing the GNSB 941F/1340R primers with the estuary sediment samples.

ISBN: 9781321867961Subjects--Topical Terms:

536250
Microbiology.

PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi .
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100 1 $a Opene, Belita. $3 3187708
245 1 0 $a PCR Analysis of Estuary Sediments from New Hampshire and Long Island Sites Using Primers Specific to Phylum Chloroflexi .
300 $a 33 p.
500 $a Source: Masters Abstracts International, Volume: 54-06.
500 $a Adviser: Jonna Coombs.
502 $a Thesis (M.S.)--Adelphi University, 2015.
520 $a Estuaries are subject to a diverse array of disturbances such as the daily and seasonal tidal cycles and the variation of the flow of freshwater into the system. These disturbances allow for the accumulation of sediments and the formation of salt marsh environments within the estuaries. Due to the physical, biological and chemical properties of these sediments, estuaries contain diverse microbial communities that exist as single cells or micro-colonies. Scientists have been successful in culturing and identifying some of the resident microbial phyla present in estuary sediments. However, there are still many more organisms that are not culturable and can only be identified using molecular techniques. The phylum Chloroflexi has a few cultured representatives that have mainly been isolated from geothermal hot springs. However, molecular evidence indicates that this phylum is abundant in diverse environmental samples, including salt marsh sediments. In this study, metagenomic DNA was extracted from sediment samples from two salt marshes; one in New Hampshire and the other in Long Island, NY, and this DNA was subjected to polymerase chain reaction using primers targeting the Chloroflexi 16S rDNA gene. The 16S primers produced amplification product for one of the two samples indicating the presence of Chloroflexi organisms. These findings will pave the way for future qPCR work utilizing the GNSB 941F/1340R primers with the estuary sediment samples.
590 $a School code: 0001.
650 4 $a Microbiology. $3 536250
650 4 $a Molecular biology. $3 517296
650 4 $a Biogeochemistry. $3 545717
650 4 $a Chemical oceanography. $3 516760
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690 $a 0307
690 $a 0425
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710 2 $a Adelphi University. $3 1017872
773 0 $t Masters Abstracts International $g 54-06(E).
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791 $a M.S.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1592289