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The effects and mitigation of lignoc...

Stoutenburg, Rosanna.

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The effects and mitigation of lignocellulosic hydrolysate inhibitors for ethanol production by Scheffersomyces (Pichia) stipitis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The effects and mitigation of lignocellulosic hydrolysate inhibitors for ethanol production by Scheffersomyces (Pichia) stipitis./
作者:	Stoutenburg, Rosanna.
面頁冊數:	189 p.
附註:	Source: Dissertation Abstracts International, Volume: 74-10(E), Section: B.
Contained By:	Dissertation Abstracts International74-10B(E).
標題:	Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3568161
ISBN:	9781303220951

The effects and mitigation of lignocellulosic hydrolysate inhibitors for ethanol production by Scheffersomyces (Pichia) stipitis.
Stoutenburg, Rosanna.

The effects and mitigation of lignocellulosic hydrolysate inhibitors for ethanol production by Scheffersomyces (Pichia) stipitis. - 189 p.

Source: Dissertation Abstracts International, Volume: 74-10(E), Section: B.

Thesis (Ph.D.)--State University of New York College of Environmental Science and Forestry, 2013.

This item is not available from ProQuest Dissertations & Theses.

The development of renewable fuel sources is crucial for meeting transportation needs in the future. Ethanol from lignocellulose has the potential to sustainably meet transportation energy needs in the immediate future, but it has been problematic because of feedstock-born inhibitory compounds that stifle fermentation. Lignocellulosic inhibitor mitigation through hydrolysate detoxification and S. stipitis strain adaptation were explored. Although detoxification treatments greatly improved the feedstock fermentability, it increases process inputs and removes fermentable sugars. Adaptation of S. stipitis to a lignocellulosic hydrolysate yielded three strains (RS01-03) that were capable of rapid fermentation with ethanol yields as great as 0.49 g ethanol/ g sugar. These hydrolysate-adapted strains were studied further for their abilities to utilize glucose and xylose and their tolerances and potential catabolism of lignocellulosic inhibitors. S. stipitis> RS01-03 exhibited improved fermentation of glucose that may be related to glucose transport. The hydrolysate-adapted strains demonstrated greater tolerances to furfural, 5-hydroxymethyl furfural, vanillic acid, and syringaldehyde and lost the ability to catabolize vanillin, vanillic acid, and benzoic acid. This result reveals that inhibitor catabolism is not likely to be a mechanism for hydrolysate tolerance, especially since catabolism would likely be repressed until carbohydrates are depleted. Conversion of furfural to furfuryl alcohol and furoic acid was monitored in S. stipitis, which revealed that the adapted strains produced furfuryl alcohol only, whereas the WT produced both products. It was discovered that furoic acid has a stronger impact on S. stipitis survival than furfuryl alcohol and therefore, the loss of furoic acid production from furfural in RS01-03 is an important adaptation that contributes to its greater tolerance in the presence of furfural. Observations of S. stipitis by light microscopy indicated no significant structural changes resulting from the presence of a lignocellulosic hydrolysate except that cell size is slightly reduced which is indicative of the stressful conditions. S. stipitis WT was observed to be pseudohyphal by light and electron microscopy and its characteristics were similar to S. cerevisiae pseudohyphae. S. stipitis WT and RS01 both exhibited extracellular matrices and flocculation, characteristics that may convey inhibitor resistance and community survival strategies, respectively.

ISBN: 9781303220951Subjects--Topical Terms:

536250
Microbiology.

The effects and mitigation of lignocellulosic hydrolysate inhibitors for ethanol production by Scheffersomyces (Pichia) stipitis.
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