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Genotypic and phenotypic characteris...

Qiao, XiaoTan.

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Genotypic and phenotypic characteristics ofp53, HER/neu and Bcl-2 in Chinese primary breast cancer.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Genotypic and phenotypic characteristics ofp53, HER/neu and Bcl-2 in Chinese primary breast cancer./
作者:	Qiao, XiaoTan.
面頁冊數:	156 p.
附註:	Source: Dissertation Abstracts International, Volume: 59-10, Section: B, page: 5319.
Contained By:	Dissertation Abstracts International59-10B.
標題:	Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9909358
ISBN:	9780599076396

Genotypic and phenotypic characteristics ofp53, HER/neu and Bcl-2 in Chinese primary breast cancer.
Qiao, XiaoTan.

Genotypic and phenotypic characteristics ofp53, HER/neu and Bcl-2 in Chinese primary breast cancer. - 156 p.

Source: Dissertation Abstracts International, Volume: 59-10, Section: B, page: 5319.

Thesis (Ph.D.)--Michigan State University, 1997.

There is a need to compare the various descriptional parameters currently used to define breast cancer with the underlying genetic alterations. The work in this dissertation addresses this need by examining paraffin embedded samples of breast cancer collected previously for routine diagnosis. Two hundred and five specimens from 178 cases of primary breast cancer in patients from China, those specimens were evaluated for clinical and histopathology features of cancer. One hundred thirty-four primary invasive breast carcinomas from this group were examined for p53, HER/neu (c-erbB-2) and Bcl-2 protein expression by immunohistochemical methods; 14.18%, 23.13% and 66.42%, respectively stained positively. p53+/HER+/Bcl-2+, p53+/HER-/Bcl-2+, p53+/HER+ and p53+/Bcl-2+ were associated with poor breast cancer prognosis. HER/neu (c-erbB-2) genes were amplified and detected by differential PCR and band intensity methods; 29.10% was positive. Sixty-four of them were evaluated for p53 gene point mutations by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP), followed by gene sequence analysis; a positivity of 6.15% was found and included 2 missense, 1 intron and 1 silent, mutations. The detected characteristics of p53 and HER/neu gene mutations, the proteins expressed, together with Bcl-2 protein accumulation, were used to define differences between two groups: the Western population (high breast cancer risk) and Chinese people (low breast cancer risk) these had similar genotypes and phenotypes except for the Chinese breast cancer patients tending to be younger, to have a higher frequency of infiltrating ductal cancer and have a longer disease free survival time. Aspects of these findings that might provide information as to the mechanisms involved in carcinogenesis are discussed.

ISBN: 9780599076396Subjects--Topical Terms:

643180
Pathology.

Genotypic and phenotypic characteristics ofp53, HER/neu and Bcl-2 in Chinese primary breast cancer.
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