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Profiling gene expression during ear...

Atallah, Nadia.

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Profiling gene expression during early gametophyte development and sex determination in Ceratopteris richardii.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Profiling gene expression during early gametophyte development and sex determination in Ceratopteris richardii./
作者:	Atallah, Nadia.
面頁冊數:	229 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-12(E), Section: B.
Contained By:	Dissertation Abstracts International76-12B(E).
標題:	Plant sciences. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3718955
ISBN:	9781321991468

Profiling gene expression during early gametophyte development and sex determination in Ceratopteris richardii.
Atallah, Nadia.

Profiling gene expression during early gametophyte development and sex determination in Ceratopteris richardii. - 229 p.

Source: Dissertation Abstracts International, Volume: 76-12(E), Section: B.

Thesis (Ph.D.)--Purdue University, 2015.

In the fern Ceratopteris richardii, every spore has the potential to develop as either a male or hermaphroditic gametophyte. Gametophyte sex is determined by a GA-like pheromone (ACE) that is secreted by hermaphrodites approximately 6 days after spore inoculation and induces male development in other juvenile gametophytes. Our goal is to better understand the genetic and molecular mechanisms involved in sex determination and to identify sex determination genes in Ceratopteris. RNA-Seq was used to create de novo transcriptome assemblies from gametophytes grown, with or without ACE, during the time that their sex is determined, and from male gametophytes in early development. We found that ACE alters the expression of 1,163 genes, including those involved in epigenetic reprogramming of the genome. This suggests that epigenetics plays an important role in the early establishment of the male program of expression. We also found that a large number of transcripts are stored in the dormant spore (18,437) and that the transcriptomes of male gametophytes early in development are incredibly dynamic. The research presented in this thesis was used to generate easily testable hypotheses and to identify candidate sex-determining genes that had been genetically characterized previously. We propose that the HERMAPHRODITIC gene encodes GID1, the ACE receptor, that the TRANSFORMER gene encodes a DELLA protein, and that the FEMINIZATION (FEM) gene encodes a MYB transcription factor. We also propose that FEM directly or indirectly blocks ACE synthesis in the male by down-regulating the expression of a gene (CPS/KS) that is essential for GA biosynthesis.

ISBN: 9781321991468Subjects--Topical Terms:

3173832
Plant sciences.

Profiling gene expression during early gametophyte development and sex determination in Ceratopteris richardii.
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