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Synthesis and evaluation of glyconan...

Jayawardena, Hapuarachchige Surangi Nelusha.

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Synthesis and evaluation of glyconanomaterials in discriminating lectins, cell surface interactions, and antimicrobial therapeutics.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Synthesis and evaluation of glyconanomaterials in discriminating lectins, cell surface interactions, and antimicrobial therapeutics./
作者:	Jayawardena, Hapuarachchige Surangi Nelusha.
面頁冊數:	176 p.
附註:	Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.
Contained By:	Dissertation Abstracts International76-04B(E).
標題:	Chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3581975
ISBN:	9781321442175

Synthesis and evaluation of glyconanomaterials in discriminating lectins, cell surface interactions, and antimicrobial therapeutics.
Jayawardena, Hapuarachchige Surangi Nelusha.

Synthesis and evaluation of glyconanomaterials in discriminating lectins, cell surface interactions, and antimicrobial therapeutics. - 176 p.

Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.

Thesis (Ph.D.)--University of Massachusetts Lowell, 2015.

This item is not available from ProQuest Dissertations & Theses.

This dissertation focuses on the synthesis and characterization of glyconanomaterials, as well as their applications in sensing and therapeutics. Underivatized carbohydrates were coupled to nanomaterials through photochemically induced coupling reaction of perfluorophenylazide (PFPA). A wide variety of carbohydrates including monosaccharides, disaccharides and oligosaccharides were conjugated to nanoparticles with high yields and efficiency to prepare glyconanomaterials. Nanoparticle scaffold for glyconanomaterials discussed in the dissertation are spherical silica (including the dye-doped fluorescent silica) and gold nanoparticles with particles sizes ranging from &sim; 20-100 nm. We have taken advantage of the multivalent presentation of carbohydrates on glyconanomaterials to study interactions with legume lectins, bacterial and mammalian cells. Finally, the novel concept of glyconanoantibiotics as an antibacterial therapeutic to inhibit drug resistant gram-negative bacteria is explored.

ISBN: 9781321442175Subjects--Topical Terms:

516420
Chemistry.

Synthesis and evaluation of glyconanomaterials in discriminating lectins, cell surface interactions, and antimicrobial therapeutics.
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500 $a Source: Dissertation Abstracts International, Volume: 76-04(E), Section: B.
500 $a Adviser: Mingdi Yan.
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506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a This dissertation focuses on the synthesis and characterization of glyconanomaterials, as well as their applications in sensing and therapeutics. Underivatized carbohydrates were coupled to nanomaterials through photochemically induced coupling reaction of perfluorophenylazide (PFPA). A wide variety of carbohydrates including monosaccharides, disaccharides and oligosaccharides were conjugated to nanoparticles with high yields and efficiency to prepare glyconanomaterials. Nanoparticle scaffold for glyconanomaterials discussed in the dissertation are spherical silica (including the dye-doped fluorescent silica) and gold nanoparticles with particles sizes ranging from &sim; 20-100 nm. We have taken advantage of the multivalent presentation of carbohydrates on glyconanomaterials to study interactions with legume lectins, bacterial and mammalian cells. Finally, the novel concept of glyconanoantibiotics as an antibacterial therapeutic to inhibit drug resistant gram-negative bacteria is explored.
520 $a We synthesized an array of gold glyconanoparticles (Au GNPs) and utilized its localized surface plasmon resonance (LSPR) to study the interactions with a few selected legume lectins. Linear discriminant analysis was employed to discriminate one legume lectin from another utilizing the LSPR behavior of the Au GNPs. All four legume lectin used in the study was readily identified in blind experiments, and classified with 100% accuracy by the discriminatory models. In another study, D-maltoheptaose (G7) conjugated glyconanoparticles (G7-NPs) were used to study the interactions with Gram-negative bacteria, Escherichia coli. The G7-NPs demonstrated intense interactions with the bacterial cell surface, but surprisingly lacked similar intensity of behavior on mammalian cells. Control studies with other saccharides demonstrated that the intensity of behavior displayed was limited to G7-NPs. In a parallel study, the intense surface interactions with G7-NPs were utilized in a novel concept of glyconanoantibiotics, where nanoparticle surface is scaffold to both an antibiotic and G7. Glyconanoantibiotics are compared side-by-side to nanoantibiotics (antibiotic-NPs), and the antibacterial efficacy was tested against three Gram-negative strains. Overall, glyconanoantibiotics performed a maximum of one log fold better than the nanoantibiotics and three log-folds better than the free drug.
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