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Matrix-assisted laser desorption ion...

Getreu, Adam.

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Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme./
作者:	Getreu, Adam.
面頁冊數:	30 p.
附註:	Source: Masters Abstracts International, Volume: 53-03.
Contained By:	Masters Abstracts International53-03(E).
標題:	Dentistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1560106
ISBN:	9781321016529

Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme.
Getreu, Adam.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme. - 30 p.

Source: Masters Abstracts International, Volume: 53-03.

Thesis (M.S.)--Temple University, 2014.

Objectives: Porphyromonas gingivalis is recognized as a major periodontal bacterial species in human periodontitis. The purpose of this study was to assess with MALDI-TOF mass spectrometry the accuracy of periodontal P. gingivalis identification with an anaerobic culture/phenotypic test scheme first proposed in 1987, and widely utilized since then in periodontal microbiology culture-based research studies.

ISBN: 9781321016529Subjects--Topical Terms:

828971
Dentistry.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme.
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100 1 $a Getreu, Adam. $3 3179288
245 1 0 $a Matrix-assisted laser desorption ionization-time of flight mass spectrometry validation of a periodontal Porphyromonas gingivalis identification scheme.
300 $a 30 p.
500 $a Source: Masters Abstracts International, Volume: 53-03.
500 $a Adviser: Thomas E. Rams.
502 $a Thesis (M.S.)--Temple University, 2014.
520 $a Objectives: Porphyromonas gingivalis is recognized as a major periodontal bacterial species in human periodontitis. The purpose of this study was to assess with MALDI-TOF mass spectrometry the accuracy of periodontal P. gingivalis identification with an anaerobic culture/phenotypic test scheme first proposed in 1987, and widely utilized since then in periodontal microbiology culture-based research studies.
520 $a Methods: 43 clinical subgingival isolates of P. gingivalis exposed to overnight refrigeration following their initial cultivation, and two other subgingival P. gingivalis subjected to refrigeration for over a one week period following their initial cultivation, were identified after anaerobic incubation on enriched Brucella blood agar based on their dark-pigmented colony morphology, lack of long-wave ultraviolet light autofluorescence, and a positive CAAM test for trypsin-like activity. Each of these putative P. gingivalis clinical isolates, along with a manufacturer-recommended bacterial test standard, comprised of Escherichia coli, and two clinical isolates of subgingival Prevotella intermedia/nigrescens , were subjected to MALDI-TOF mass spectrometry analysis using a bench top mass spectrometer, and Bruker FlexControl 3.0 and MALDI Biotyper 3.1 software (Bruker Daltonics, Billerica, MA, USA), capable of definitively identifying 4,613 different bacterial species, including P. gingivalis . A MALDI Biotyper score of ≥ 1.7 was utilized for definitive P. gingivalis identification. Kappa analysis determined the extent of P. gingivalis identification concordance between the phenotypic identification anaerobic culture/CAAM test scheme, and MALDI-TOF mass spectrometry test results, for the 43 P. gingivalis clinical isolates exposed to only overnight refrigeration.
520 $a Results: 41 (95.3%) of the 43 presumptive subgingival P. gingivalis clinical isolates exposed to only overnight refrigeration following their initial cultivation were definitively identified as P. gingivalis in MALDI-TOF mass spectrometry analysis, with MALDI Biotyper scores of ≥ 1.7. A kappa value = 0.995, indicating excellent agreement beyond chance alone, was found for the relationship between P. gingivalis identification determined by the anaerobic culture/CAAM test scheme, and MALDI-TOF mass spectrometry test results. For the two presumptive P. gingivalis clinical isolates exposed to overnight refrigeration not definitively identified with MALDI-TOF mass spectrometry analysis, P. gingivalis was still the first species listed as their possible identification by the MALDI Biotyper software. The two subgingival P. gingivalis clinical isolates subjected to refrigeration for over a one week period following their initial cultivation were not definitively identified in MALDI-TOF mass spectrometry analysis. The two clinical isolates phenotypically-identified as P. intermedia/nigrescens were definitively identified in MALDI-TOF mass spectrometry analysis as Prevotella nigrescens.
520 $a Conclusions: These findings document, for the first time with MALDI-TOF mass spectrometry, the accuracy of periodontal P. gingivalis identification based on darkpigmented colony morphology, lack of long-wave ultraviolet light autofluorescence, and a positive CAAM test for trypsin-like activity. 95.3% of 43 presumptive subgingival P. gingivalis clinical isolates recognized with a phenotypic/biochemical-based identification scheme, and exposed to only overnight refrigeration following their initial cultivation, were definitively identified as P. gingivalis in MALDI-TOF mass spectrometry analysis. These findings provide validation for the continued use of prior and current research data employing a phenotypic/biochemical-based identification scheme for periodontal P. gingivalis. (Abstract shortened by UMI.).
590 $a School code: 0225.
650 4 $a Dentistry. $3 828971
650 4 $a Microbiology. $3 536250
690 $a 0567
690 $a 0410
710 2 $a Temple University. $b Oral Biology. $3 3179289
773 0 $t Masters Abstracts International $g 53-03(E).
790 $a 0225
791 $a M.S.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1560106