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A role for UV-B -induced DNA damage ...

Biever, Jessica J.

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A role for UV-B -induced DNA damage in photomorphogenic responses in etiolated Arabidopsis seedlings.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A role for UV-B -induced DNA damage in photomorphogenic responses in etiolated Arabidopsis seedlings./
作者:	Biever, Jessica J.
面頁冊數:	89 p.
附註:	Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.
Contained By:	Dissertation Abstracts International75-06B(E).
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3612686
ISBN:	9781303744471

A role for UV-B -induced DNA damage in photomorphogenic responses in etiolated Arabidopsis seedlings.
Biever, Jessica J.

A role for UV-B -induced DNA damage in photomorphogenic responses in etiolated Arabidopsis seedlings. - 89 p.

Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.

Thesis (Ph.D.)--University of Minnesota, 2014.

Ultraviolet (UV) radiation is a constituent of sunlight that influences plant morphology and growth. It induces photomorphogenic responses but also causes damage to DNA. Plant responses to DNA damage caused by UV-B light are often categorized as general mechanisms that get activated by other environmental stresses. Photodimers are formed through the direct absorption of UV-B light by DNA and are removed, in part, by nucleotide excision repair (NER). UV-B irradiation resulted in the accumulation of the two most common photodimers, cyclobutane pyrimidine dimers (CPDs) and pyrimidine-(6,4)-pyrimidinone dimers (6,4PPs), in etiolated wild type (wt) Arabidopsis seedlings. Arabidopsis mutants of the endonucleases that function in NER, xpf-3 and uvr1-1, show hypersensitivity to UV-B (280-320 nm) in terms of hypocotyl growth inhibition. I hypothesized that the accumulation of UV-B-induced photodimers was responsible for the hypocotyl growth phenotype of these NER mutants after UV-B irradiation. It was also predicted that the accumulation of photodimers could ultimately trigger signaling pathways that result in cell-cycle arrest through stalled replication sites or double-strand breaks. This was tested using the suppressor of gamma 1 (sog1-1) mutant, which lacks a transcription factor responsible for gene induction and cell-cycle arrest after gamma irradiation, and a Col-0 line containing a CYCB1;1-GUS reporter construct. CYCB1;1 encodes a cyclin that accumulates in response to cell-cycle arrest at the G2/M transition. The main conclusion from this work is that hypocotyl growth inhibition induced by UV-B light in etiolated Arabidopsis seedlings, which is a classic photomorphogenic response, is influenced by signals originating from UV-B light absorption by DNA that lead to cell-cycle arrest. Furthermore, this process is shown to occur independently of UVR8 and its signaling pathway responsible for CHS induction. This work also demonstrates that UV-B-induced DNA damage can be responsible for specific photomorphogenic responses, at least in etiolated Arabidopsis seedlings, and does not simply induce general stress responses.

ISBN: 9781303744471Subjects--Topical Terms:

1017825
Biology, Botany.

A role for UV-B -induced DNA damage in photomorphogenic responses in etiolated Arabidopsis seedlings.
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