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Nonlinear optical techniques for ima...

Farrar, Matthew John.

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Nonlinear optical techniques for imaging and manipulating the mouse central nervous system.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Nonlinear optical techniques for imaging and manipulating the mouse central nervous system./
Author:	Farrar, Matthew John.
Description:	325 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.
Contained By:	Dissertation Abstracts International74-03B(E).
Subject:	Physics, Optics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3531021
ISBN:	9781267717016

Nonlinear optical techniques for imaging and manipulating the mouse central nervous system.
Farrar, Matthew John.

Nonlinear optical techniques for imaging and manipulating the mouse central nervous system. - 325 p.

Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.

Thesis (Ph.D.)--Cornell University, 2012.

The spinal cord of vertebrates serves as the conduit for somatosensory information and motor control, as well as being the locus of neural circuits that govern fast reflexes and patterned behaviors, such as walking in mammals or swimming in fish. Consequently, pathologies of the spinal cord --such as spinal cord injury (SCI)-- lead to loss of motor control and sensory perception, with accompanying decline in life expectancy and quality of life. Despite the devastating effects of these diseases, few therapies exist to substantially ameliorate patient outcome.

ISBN: 9781267717016Subjects--Topical Terms:

1018756
Physics, Optics.

Nonlinear optical techniques for imaging and manipulating the mouse central nervous system.
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020 $a 9781267717016
035 $a (MiAaPQ)AAI3531021
035 $a AAI3531021
040 $a MiAaPQ $c MiAaPQ
100 1 $a Farrar, Matthew John. $3 2102398
245 1 0 $a Nonlinear optical techniques for imaging and manipulating the mouse central nervous system.
300 $a 325 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-03(E), Section: B.
500 $a Adviser: Chris Schaffer.
502 $a Thesis (Ph.D.)--Cornell University, 2012.
520 $a The spinal cord of vertebrates serves as the conduit for somatosensory information and motor control, as well as being the locus of neural circuits that govern fast reflexes and patterned behaviors, such as walking in mammals or swimming in fish. Consequently, pathologies of the spinal cord --such as spinal cord injury (SCI)-- lead to loss of motor control and sensory perception, with accompanying decline in life expectancy and quality of life. Despite the devastating effects of these diseases, few therapies exist to substantially ameliorate patient outcome.
520 $a In part, studies of spinal cord pathology have been limited by the inability to perform in vivo imaging at the level of cellular processes. The focus of this thesis is to present the underlying theory for and demonstration of novel multi-photon microscopy (MPM) and optical manipulation techniques as they apply to studies the mouse central nervous system (CNS), with an emphasis on the spinal cord. The scientific findings which have resulted from the implementation of these techniques are also presented.
520 $a In particular, we have demonstrated that third harmonic generation is a dye-free method of imaging CNS myelin, a fundamental constituent of the spinal cord that is difficult to label using exogenous dyes and/or transgenic constructs. Since gaining optical access to the spinal cord is a prerequisite for spinal cord imaging, we review our development of a novel spinal cord imaging chamber and surgical procedure which allowed us to image for multiple weeks following implantation without the need for repeated surgeries. We also have used MPM to characterize spinal venous blood flow before and after point occlusions. We review a novel nonlinear microscopy technique that may serve to show optical interfaces in three dimensions inside scattering tissue. Finally, we discuss a model and show results of optoporation, a means of transfecting cells with genetic constructs. Brief reviews of MPM and SCI are also presented.
590 $a School code: 0058.
650 4 $a Physics, Optics. $3 1018756
650 4 $a Biology, Neurobiology. $3 1681328
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0752
690 $a 0421
690 $a 0541
710 2 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 74-03B(E).
790 $a 0058
791 $a Ph.D.
792 $a 2012
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3531021