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The effect of freezing rate on strip...

Frankel, Tyler Edward.

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The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	The effect of freezing rate on striped bass (morone saxatilis) spermatozoa./
作者:	Frankel, Tyler Edward.
面頁冊數:	83 p.
附註:	Source: Masters Abstracts International, Volume: 52-03.
Contained By:	Masters Abstracts International52-03(E).
標題:	Agriculture, Animal Culture and Nutrition. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1547722
ISBN:	9781303498855

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.
Frankel, Tyler Edward.

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa. - 83 p.

Source: Masters Abstracts International, Volume: 52-03.

Thesis (M.S.)--University of Maryland, College Park, 2013.

Several studies have attempted to determine the optimal freezing rate for the cryopreservation of striped bass spermatozoa. In this study, the effects of freezing rate (-10, -15, -20 and -40oC/min) on sperm quality was examined utilizing Sybr- 14/Propidium Iodide staining to confirm membrane integrity, a luciferin-luciferase assay to estimate ATP concentration and a Hamilton-Thorne CEROS(TM) system to characterize sperm motion. Males (n=12) were sampled once a week for five weeks. Samples were extended, cryo-protected using a 7.5% (v/v) dimethyl sulfoxide solution, and frozen using a controlled-rate freezer. Samples were stored in liquid nitrogen for 49 days and re-evaluated post-thaw. Sperm cryopreserved at -40oC per minute resulted in: total motility (10.06%), progressive motility (7.14%), ATP concentration (0.86 pmol/million cells), and sperm viability (56.5%); which were greater (P < 0.05) than the slower rates. Results demonstrate that -40oC/minute was the optimal freezing rate among those tested for the cryopreservation of striped bass spermatozoa.

ISBN: 9781303498855Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

The effect of freezing rate on striped bass (morone saxatilis) spermatozoa.
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