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Side population sorting separates su...

von Furstenberg, Richard Joseph.

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Side population sorting separates subfractions of intestinal stem cells.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Side population sorting separates subfractions of intestinal stem cells./
作者:	von Furstenberg, Richard Joseph.
面頁冊數:	45 p.
附註:	Source: Masters Abstracts International, Volume: 51-06.
Contained By:	Masters Abstracts International51-06(E).
標題:	Biology, Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1538154
ISBN:	9781303109065

Side population sorting separates subfractions of intestinal stem cells.
von Furstenberg, Richard Joseph.

Side population sorting separates subfractions of intestinal stem cells. - 45 p.

Source: Masters Abstracts International, Volume: 51-06.

Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2013.

The existence of intestinal stem cells (ISCs) which generate the epithelium has been recognized for over three decades. Recent work suggests there are two distinct ISC populations: active ISCs which are important for epithelial homeostasis, and quiescent ISCs that drive proliferation after injury. Although quiescent ISCs may be of greater therapeutic use, to date, isolation of this population has been achieved only with fluorescent reporter mice. My goal was to identify an isolation method for both ISC populations that can be used in wild-type mice and eventually humans. In the following experiments I use side population (SP) sorting to demonstrate that the USP: contains reporter cells of the active ISC, is highly proliferative by S phase analysis, and expresses high levels of the known active ISC transcripts. In contrast the LSP is: non-proliferative, de-enriched for active ISC transcripts, and enriched for those of quiescent ISCs.

ISBN: 9781303109065Subjects--Topical Terms:

1017816
Biology, Physiology.

Side population sorting separates subfractions of intestinal stem cells.
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502 $a Thesis (M.S.)--The University of North Carolina at Chapel Hill, 2013.
520 $a The existence of intestinal stem cells (ISCs) which generate the epithelium has been recognized for over three decades. Recent work suggests there are two distinct ISC populations: active ISCs which are important for epithelial homeostasis, and quiescent ISCs that drive proliferation after injury. Although quiescent ISCs may be of greater therapeutic use, to date, isolation of this population has been achieved only with fluorescent reporter mice. My goal was to identify an isolation method for both ISC populations that can be used in wild-type mice and eventually humans. In the following experiments I use side population (SP) sorting to demonstrate that the USP: contains reporter cells of the active ISC, is highly proliferative by S phase analysis, and expresses high levels of the known active ISC transcripts. In contrast the LSP is: non-proliferative, de-enriched for active ISC transcripts, and enriched for those of quiescent ISCs.
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