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Whole-animal to molecular studies of...

Guffey, Samuel Clark.

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Whole-animal to molecular studies of solute transport in fishes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Whole-animal to molecular studies of solute transport in fishes./
Author:	Guffey, Samuel Clark.
Description:	158 p.
Notes:	Source: Masters Abstracts International, Volume: 52-05.
Contained By:	Masters Abstracts International52-05(E).
Subject:	Biology, Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MS25338
ISBN:	9780499253385

Whole-animal to molecular studies of solute transport in fishes.
Guffey, Samuel Clark.

Whole-animal to molecular studies of solute transport in fishes. - 158 p.

Source: Masters Abstracts International, Volume: 52-05.

Thesis (M.S.)--University of Alberta (Canada), 2013.

I have examined solute transport in two fishes at the whole-animal, tissue, and molecular levels. In the Pacific hagfish, I measured the kinetics of phosphate absorption in isolated gut, skin, and gill preparations, and implicated an NaPi-II transporter based on RT-PCR results and the kinetics of phosphate uptake. In a second series of experiments, subjecting Pacific dogfish to reduced salinity revealed that plasma sodium and chloride homeostasis are maintained. Therefore these ions are not a limiting factor in reduced salinity; however, urea efflux significantly increased over the 48 hour exposure. Finally, I investigated the molecular physiology of sodium homeostasis by cloning Na +/H+ Exchanger [NHE] isoforms 2 and 3 from the dogfish gill and expressing them in NHE-deficient AP-1 cell lines. The sensitivity to inhibitors was found to be different from mammalian NHEs, revealing appropriate concentrations to use for elasmobranchs and other fishes, and allowing future mechanistic studies to be conducted.

ISBN: 9780499253385Subjects--Topical Terms:

1017816
Biology, Physiology.

Whole-animal to molecular studies of solute transport in fishes.
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020 $a 9780499253385
035 $a (MiAaPQ)AAIMS25338
035 $a AAIMS25338
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100 1 $a Guffey, Samuel Clark. $3 2101522
245 1 0 $a Whole-animal to molecular studies of solute transport in fishes.
300 $a 158 p.
500 $a Source: Masters Abstracts International, Volume: 52-05.
500 $a Adviser: Greg Goss.
502 $a Thesis (M.S.)--University of Alberta (Canada), 2013.
520 $a I have examined solute transport in two fishes at the whole-animal, tissue, and molecular levels. In the Pacific hagfish, I measured the kinetics of phosphate absorption in isolated gut, skin, and gill preparations, and implicated an NaPi-II transporter based on RT-PCR results and the kinetics of phosphate uptake. In a second series of experiments, subjecting Pacific dogfish to reduced salinity revealed that plasma sodium and chloride homeostasis are maintained. Therefore these ions are not a limiting factor in reduced salinity; however, urea efflux significantly increased over the 48 hour exposure. Finally, I investigated the molecular physiology of sodium homeostasis by cloning Na +/H+ Exchanger [NHE] isoforms 2 and 3 from the dogfish gill and expressing them in NHE-deficient AP-1 cell lines. The sensitivity to inhibitors was found to be different from mammalian NHEs, revealing appropriate concentrations to use for elasmobranchs and other fishes, and allowing future mechanistic studies to be conducted.
590 $a School code: 0351.
650 4 $a Biology, Physiology. $3 1017816
650 4 $a Agriculture, Fisheries and Aquaculture. $3 1020913
650 4 $a Biology, Evolution and Development. $3 1675612
650 4 $a Biology, Botany. $3 1017825
690 $a 0719
690 $a 0792
690 $a 0412
690 $a 0309
710 2 $a University of Alberta (Canada). $b Biological Sciences. $3 2101523
773 0 $t Masters Abstracts International $g 52-05(E).
790 $a 0351
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MS25338