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Microbial community composition of m...

Killea, Lauren M.

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Microbial community composition of marine biofilms in a northern temperate estuary.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Microbial community composition of marine biofilms in a northern temperate estuary./
Author:	Killea, Lauren M.
Description:	147 p.
Notes:	Source: Masters Abstracts International, Volume: 52-06.
Contained By:	Masters Abstracts International52-06(E).
Subject:	Biology, Oceanography. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1555660
ISBN:	9781303886881

Microbial community composition of marine biofilms in a northern temperate estuary.
Killea, Lauren M.

Microbial community composition of marine biofilms in a northern temperate estuary. - 147 p.

Source: Masters Abstracts International, Volume: 52-06.

Thesis (M.S.)--University of Rhode Island, 2014.

Marine biofilms are microbial aggregates that ubiquitously develop on substrates in seawater. Biofilms are not simple layers of microorganisms adhering to a surface followed by new organisms growing on top, but they instead have a complex developmental process making biofilms dynamic, diverse and functional communities. The negative effects of biofilms on ships, underwater cables and pipelines have spawned research in antifouling approaches; however, little is known about their development in a northern temperate estuary, such as Narragansett Bay. The goal of this study was to investigate the first steps in biofouling in this area by assessing biofilm biomass through chlorophyll, carbon, nitrogen, total DNA extractions and percent biomass coverage, as well as bacterial biofilm community composition through the use of a molecular technique, the automated ribosomal intergenic spacer analysis (ARISA). Comparisons were made between biofilms on control surfaces and surfaces treated with a commercial foul-release coating, between biofilms grown in the summer and winter seasons, as well as over a 90-day immersion time. Biofilm biomass data revealed no overall significant differences between seasons or across surface types; however, immersion time had a significant effect as biomass tended to accumulate over time. Bacterial community composition obtained from ARISA profiles was influenced by immersion time, as communities separated out into 'early,' 'mid,' and 'late' groupings. It was also influenced by season as well as surface type.

ISBN: 9781303886881Subjects--Topical Terms:

783691
Biology, Oceanography.

Microbial community composition of marine biofilms in a northern temperate estuary.
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020 $a 9781303886881
035 $a (MiAaPQ)AAI1555660
035 $a AAI1555660
040 $a MiAaPQ $c MiAaPQ
100 1 $a Killea, Lauren M. $3 2101393
245 1 0 $a Microbial community composition of marine biofilms in a northern temperate estuary.
300 $a 147 p.
500 $a Source: Masters Abstracts International, Volume: 52-06.
500 $a Adviser: Lucie Maranda.
502 $a Thesis (M.S.)--University of Rhode Island, 2014.
520 $a Marine biofilms are microbial aggregates that ubiquitously develop on substrates in seawater. Biofilms are not simple layers of microorganisms adhering to a surface followed by new organisms growing on top, but they instead have a complex developmental process making biofilms dynamic, diverse and functional communities. The negative effects of biofilms on ships, underwater cables and pipelines have spawned research in antifouling approaches; however, little is known about their development in a northern temperate estuary, such as Narragansett Bay. The goal of this study was to investigate the first steps in biofouling in this area by assessing biofilm biomass through chlorophyll, carbon, nitrogen, total DNA extractions and percent biomass coverage, as well as bacterial biofilm community composition through the use of a molecular technique, the automated ribosomal intergenic spacer analysis (ARISA). Comparisons were made between biofilms on control surfaces and surfaces treated with a commercial foul-release coating, between biofilms grown in the summer and winter seasons, as well as over a 90-day immersion time. Biofilm biomass data revealed no overall significant differences between seasons or across surface types; however, immersion time had a significant effect as biomass tended to accumulate over time. Bacterial community composition obtained from ARISA profiles was influenced by immersion time, as communities separated out into 'early,' 'mid,' and 'late' groupings. It was also influenced by season as well as surface type.
590 $a School code: 0186.
650 4 $a Biology, Oceanography. $3 783691
650 4 $a Biology, Molecular. $3 1017719
690 $a 0416
690 $a 0307
710 2 $a University of Rhode Island. $b Oceanography. $3 2101394
773 0 $t Masters Abstracts International $g 52-06(E).
790 $a 0186
791 $a M.S.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1555660