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Development of microbubbles in polyd...

Giang, Ut-Binh Thi.

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Development of microbubbles in polydimethylsiloxane as a screening tool for correlating the metastatic potential of cancer cells via their morphology, motility, and clonogenic potential.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of microbubbles in polydimethylsiloxane as a screening tool for correlating the metastatic potential of cancer cells via their morphology, motility, and clonogenic potential./
作者:	Giang, Ut-Binh Thi.
面頁冊數:	233 p.
附註:	Source: Dissertation Abstracts International, Volume: 75-02(E), Section: B.
Contained By:	Dissertation Abstracts International75-02B(E).
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3600379
ISBN:	9781303504747

Development of microbubbles in polydimethylsiloxane as a screening tool for correlating the metastatic potential of cancer cells via their morphology, motility, and clonogenic potential.
Giang, Ut-Binh Thi.

Development of microbubbles in polydimethylsiloxane as a screening tool for correlating the metastatic potential of cancer cells via their morphology, motility, and clonogenic potential. - 233 p.

Source: Dissertation Abstracts International, Volume: 75-02(E), Section: B.

Thesis (Ph.D.)--University of Rochester, 2013.

Developing new methods that can determine the metastatic potential of cells as it relates to the progression of malignant cancers is a key step towards achieving more effective diagnosis and therapeutic treatments of cancer. In vitro methods, such as three dimensional cell cultures, developed to recapitulate the cellular microenvironment have been crucial for the investigative studies of in vivo cell behavior. These 3D cell cultures provide a unique cellular organization that has been shown to exhibit characteristics that are significantly different from standard 2D cultures. In this thesis work, we have developed a novel gas expansion molding (GEM) technique that creates microbubble wells that we exploit for cell culture. Microbubble (MB) wells are spherical structures formed in polydimethylsiloxane (PDMS) with 100 &mgr;m opening and 250 &mgr;m diameter. GEM can form homogeneously sized MB wells over a large area, however, there are key parameters that must be considered. We have done extensive studies to verify the key design parameters that affect microbubble formation efficiency (MBFE) and size. These factors include (1) the geometry of the mold features, (2) the spatial arrangement of the mold openings, (3) PDMS curing temperature, (4) wafer hydrophobicity, (5) the composition of the residual gases in the PDMS pre-polymer mixture, and (6) PDMS thickness. Utilizing these MBs for cell culture, we have shown that cells cultured in them undergo morphological transformations such as epithelial-to-mesenchymal-like transitions (EMT) that is due to its unique spherical geometry. We exploit the migratory characteristics of the mesenchymal-like phenotype using the MB's physical architecture to provide a qualitative measurement of the ability of melanoma cells to metastasize from a seeded site. The ability to verify the metastatic potential of cancer cells by growth characteristics is advantageous in that the innate behavior of the cells can be observed. In this thesis work, we show that the pre-determined metastatic potential of three cell lines (A375.P, A375.MA1, and A375.MA2) can be differentiated via three metrics when seeded in MB wells in PDMS including the cells' morphological variation, their radial migration from the MB wells, and their clonogenic potential. This system has the potential to be a more physiologically relevant model to recapitulate the proliferation and migration of metastatic melanoma cells originating from a single seeded site.

ISBN: 9781303504747Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Development of microbubbles in polydimethylsiloxane as a screening tool for correlating the metastatic potential of cancer cells via their morphology, motility, and clonogenic potential.
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