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A Novel Pulse-Chase Paradigm to Visu...

Al-Bassam, Sarmad.

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A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons./
Author:	Al-Bassam, Sarmad.
Description:	86 p.
Notes:	Source: Dissertation Abstracts International, Volume: 74-10(E), Section: B.
Contained By:	Dissertation Abstracts International74-10B(E).
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3563741
ISBN:	9781303122309

A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons.
Al-Bassam, Sarmad.

A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons. - 86 p.

Source: Dissertation Abstracts International, Volume: 74-10(E), Section: B.

Thesis (Ph.D.)--University of Southern California, 2013.

In neurons transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here we adapt a novel pulse chase system that allows synchronous release of exogenous transmembrane proteins from the endoplasmic reticulum using FKBP12 and Rapamycin. We demonstrate proof-of-concept and establish protein trafficking controls in incremental steps. We demonstrate the utility of this approach in studying protein trafficking and establish parameters for analysis of time-lapse images. We implement this novel pulse-chase strategy to track the movements of post-Golgi transport vesicles. Surprisingly, we found that post-Golgi vesicles carrying dendritic proteins were equally likely to enter axons and dendrites. However, once such vesicles entered the axon they very rarely moved beyond the axon initial segment, but instead either halted or reversed direction in an actin and Myosin Va-dependent manner. In contrast, vesicles carrying either an axonal or a nonspecifically localized protein only rarely halted or reversed and instead generally proceeded to the distal axon. Thus, our results are consistent with the axon initial segment behaving as a vesicle filter that mediates the differential trafficking of transport vesicles.

ISBN: 9781303122309Subjects--Topical Terms:

1017686
Biology, Cell.

A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons.
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020 $a 9781303122309
035 $a (MiAaPQ)AAI3563741
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100 1 $a Al-Bassam, Sarmad. $3 2094932
245 1 2 $a A Novel Pulse-Chase Paradigm to Visualize the Trafficking of Transport Vesicles in Neurons.
300 $a 86 p.
500 $a Source: Dissertation Abstracts International, Volume: 74-10(E), Section: B.
500 $a Adviser: Don B. Arnold.
502 $a Thesis (Ph.D.)--University of Southern California, 2013.
520 $a In neurons transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here we adapt a novel pulse chase system that allows synchronous release of exogenous transmembrane proteins from the endoplasmic reticulum using FKBP12 and Rapamycin. We demonstrate proof-of-concept and establish protein trafficking controls in incremental steps. We demonstrate the utility of this approach in studying protein trafficking and establish parameters for analysis of time-lapse images. We implement this novel pulse-chase strategy to track the movements of post-Golgi transport vesicles. Surprisingly, we found that post-Golgi vesicles carrying dendritic proteins were equally likely to enter axons and dendrites. However, once such vesicles entered the axon they very rarely moved beyond the axon initial segment, but instead either halted or reversed direction in an actin and Myosin Va-dependent manner. In contrast, vesicles carrying either an axonal or a nonspecifically localized protein only rarely halted or reversed and instead generally proceeded to the distal axon. Thus, our results are consistent with the axon initial segment behaving as a vesicle filter that mediates the differential trafficking of transport vesicles.
590 $a School code: 0208.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Nanoscience. $3 587832
690 $a 0379
690 $a 0565
710 2 $a University of Southern California. $b Biology. $3 2094933
773 0 $t Dissertation Abstracts International $g 74-10B(E).
790 $a 0208
791 $a Ph.D.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3563741