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Developing white ash with resistance...

Palla, Kaitlin J.

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Developing white ash with resistance to the emerald ash borer.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Developing white ash with resistance to the emerald ash borer./
作者:	Palla, Kaitlin J.
面頁冊數:	124 p.
附註:	Source: Masters Abstracts International, Volume: 52-03.
Contained By:	Masters Abstracts International52-03(E).
標題:	Agriculture, Forestry and Wildlife. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1546993
ISBN:	9781303477003

Developing white ash with resistance to the emerald ash borer.
Palla, Kaitlin J.

Developing white ash with resistance to the emerald ash borer. - 124 p.

Source: Masters Abstracts International, Volume: 52-03.

Thesis (M.S.)--Purdue University, 2013.

Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 microM 6-benzyladenine (BA) and 0.5 microM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 microM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 microM BA, 0.5 microM TDZ, 50 mg L-1 adenine sulfate, 10% coconut water, 30 mg L-1 kanamycin, and 500 mg L-1 timentin. The presence of cry8D2 and nptII were confirmed by polymerase chain reaction for all four transgenic lines. Transgenic white ash microshoots are being multiplied for use in future feeding trials to determine potential efficacy of the cry8D2 gene against emerald ash borer attack.

ISBN: 9781303477003Subjects--Topical Terms:

783690
Agriculture, Forestry and Wildlife.

Developing white ash with resistance to the emerald ash borer.
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