東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Genetic analysis of the promoter reg...

Alzahrani, Khaloud.

FindBook

Google Book

Amazon

博客來

Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens./
作者:	Alzahrani, Khaloud.
面頁冊數:	52 p.
附註:	Source: Masters Abstracts International, Volume: 51-05.
Contained By:	Masters Abstracts International51-05(E).
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1536288
ISBN:	9781303044335

Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens.
Alzahrani, Khaloud.

Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens. - 52 p.

Source: Masters Abstracts International, Volume: 51-05.

Thesis (M.S.)--University of Arkansas, 2013.

Pulmonary Arterial Hypertension (PAH) is a sever disorder characterized by high pulmonary artery pressure and pulmonary vascular resistance along with specific histological changes. Idiopathic PAH is a human disease of unknown origin that showed common characteristics to PHS/ascites in chicken. PHS/ascites is a condition found in broilers causing right ventricular hypertrophy, valvular insufficiency, increased venous pressure, variable liver changes, accumulation of fluids in the abdominal cavity, and finally lung and heart failure. Currently, there is no acceptable animal model for human PAH. Our group has been validating the chicken as the medical animal model for human PAH, thus we have mapped chromosomal regions associated with susceptibility to ascites. The region on Gga9 contains the serotonin receptor (5HT2B) gene which showed linkage disequilibrium with respect to ascites susceptibility. Sequence data obtained from a previous research work for the 5HT2B gene from several SUS and RES lines identified a C/T SNP on the 3rd exon that result in a silent mutation and had no effect on protein production or structure. My research project was to extend our sequence analysis to the upstream promoter regions of 5HT2B to identify any sequence polymorphisms of several DNA samples from both SUS and RES lines. I identified 15 polymorphisms SNPs present in the 5HT2B promoter region. Three SNPs were found to be in a complete linkage defining alternative alleles and have a potential ability in affecting transcription factors binding sites. A TaqMan Assay was then developed for these particular SNPs used to SNPlotype a large collection of DNA samples from birds previously phenotyped for ascites susceptibility in the hypobaric chamber challenged. Statistical analyses of the SNPlotyped data support the association of particular genotypes/haplotypes of the 5HT2B gene with resistance to ascites especially in females. Identifying the underlining genetics of ascites syndrome will lead to genetic selection to reduce ascites incidence in chicken and further establish chicken as the medical animal model for human PAH.

ISBN: 9781303044335Subjects--Topical Terms:

1017719
Biology, Molecular.

Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens.
LDR:03187nam 2200313 4500 001 1957092
005 20131112081656.5
008 150210s2013 ||||||||||||||||| ||||| d
020 $a 9781303044335
035 $a (UMI)AAI1536288
035 $a AAI1536288
040 $a UMI $c UMI
100 1 $a Alzahrani, Khaloud. $3 2091894
245 1 0 $a Genetic analysis of the promoter region of the serotonin receptor 5HT2B and its contribution to pulmonary hypertension syndrome in broiler-type chickens.
300 $a 52 p.
500 $a Source: Masters Abstracts International, Volume: 51-05.
500 $a Adviser: Douglas Rhoads.
502 $a Thesis (M.S.)--University of Arkansas, 2013.
520 $a Pulmonary Arterial Hypertension (PAH) is a sever disorder characterized by high pulmonary artery pressure and pulmonary vascular resistance along with specific histological changes. Idiopathic PAH is a human disease of unknown origin that showed common characteristics to PHS/ascites in chicken. PHS/ascites is a condition found in broilers causing right ventricular hypertrophy, valvular insufficiency, increased venous pressure, variable liver changes, accumulation of fluids in the abdominal cavity, and finally lung and heart failure. Currently, there is no acceptable animal model for human PAH. Our group has been validating the chicken as the medical animal model for human PAH, thus we have mapped chromosomal regions associated with susceptibility to ascites. The region on Gga9 contains the serotonin receptor (5HT2B) gene which showed linkage disequilibrium with respect to ascites susceptibility. Sequence data obtained from a previous research work for the 5HT2B gene from several SUS and RES lines identified a C/T SNP on the 3rd exon that result in a silent mutation and had no effect on protein production or structure. My research project was to extend our sequence analysis to the upstream promoter regions of 5HT2B to identify any sequence polymorphisms of several DNA samples from both SUS and RES lines. I identified 15 polymorphisms SNPs present in the 5HT2B promoter region. Three SNPs were found to be in a complete linkage defining alternative alleles and have a potential ability in affecting transcription factors binding sites. A TaqMan Assay was then developed for these particular SNPs used to SNPlotype a large collection of DNA samples from birds previously phenotyped for ascites susceptibility in the hypobaric chamber challenged. Statistical analyses of the SNPlotyped data support the association of particular genotypes/haplotypes of the 5HT2B gene with resistance to ascites especially in females. Identifying the underlining genetics of ascites syndrome will lead to genetic selection to reduce ascites incidence in chicken and further establish chicken as the medical animal model for human PAH.
590 $a School code: 0011.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
690 $a 0307
690 $a 0369
710 2 $a University of Arkansas. $b Cell & Molecular Biology. $3 2091895
773 0 $t Masters Abstracts International $g 51-05(E).
790 1 0 $a Rhoads, Douglas, $e advisor
790 1 0 $a Anthony, Nicholas $e committee member
790 1 0 $a McNabb, David $e committee member
790 $a 0011
791 $a M.S.
792 $a 2013
793 $a Arabic
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1536288