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Structural and functional analyses o...

Deng, Xiangning.

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Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes./
作者:	Deng, Xiangning.
面頁冊數:	132 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-02, Section: B, page: 0488.
Contained By:	Dissertation Abstracts International60-02B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9918863
ISBN:	059918003X

Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes.
Deng, Xiangning.

Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes. - 132 p.

Source: Dissertation Abstracts International, Volume: 60-02, Section: B, page: 0488.

Thesis (Ph.D.)--University of Delaware, 1999.

This research is focused on the role of the mouse Spam1 gene, which encodes a glycosylphosphatidylinositol (GPI)-linked sperm surface protein with hyaluronidase activity, in the transmission ratio distortion (TRD) and its age modulating effect in Rb(6.16) and Rb(6.15) translocation heterozygotes. The candidacy of Spam1 as the gene responsible for the dysfunction in the Rb-bearing sperm is supported by its map location at proximal MMU 6, a genomic alteration at the locus in the Rb(6.16) DNA, and the reduced Spam1 hyaluronidase activity in sperm bearing either Rb(6.16) or Rb(6.15). The reduction of hyaluronidase activity involves different mechanisms in the two Rbs. For Rb(6.16)-bearing mice, the reduction is due to both a quantitative and a qualitative defect of Spam1; while for Rb(6.15) only a qualitative defect reduces the activity.

ISBN: 059918003XSubjects--Topical Terms:

1017719
Biology, Molecular.

Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes.
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245 1 0 $a Structural and functional analyses of mousespam1 support its role in the transmission ratio distortion (TRD) and age-modulating effect in Rb(6.16) and Rb(6.15) heterozygotes.
300 $a 132 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-02, Section: B, page: 0488.
500 $a Professor in charge: Patricia A. DeLeon.
502 $a Thesis (Ph.D.)--University of Delaware, 1999.
520 $a This research is focused on the role of the mouse Spam1 gene, which encodes a glycosylphosphatidylinositol (GPI)-linked sperm surface protein with hyaluronidase activity, in the transmission ratio distortion (TRD) and its age modulating effect in Rb(6.16) and Rb(6.15) translocation heterozygotes. The candidacy of Spam1 as the gene responsible for the dysfunction in the Rb-bearing sperm is supported by its map location at proximal MMU 6, a genomic alteration at the locus in the Rb(6.16) DNA, and the reduced Spam1 hyaluronidase activity in sperm bearing either Rb(6.16) or Rb(6.15). The reduction of hyaluronidase activity involves different mechanisms in the two Rbs. For Rb(6.16)-bearing mice, the reduction is due to both a quantitative and a qualitative defect of Spam1; while for Rb(6.15) only a qualitative defect reduces the activity.
520 $a During epididymal transit of sperm, both biophysical and biochemical maturation of Spam1 were shown to occur. Spam1 is uniformly distributed over the entire head surface of immature (caput) sperm; but localized to the anterior and posterior regions of the head of mature (caudal) cells. Biochemical maturation of Spam1 is associated with an increase in hyaluronidase activity in caudal compared to caput sperm and occurs via progressive N-linked deglycosylation, which also occurs after sperm undergo the acrosome reaction. Caudal sperm were also shown to have significantly higher levels of Spam1.
520 $a Hitherto, Spam1 is known to be testis-specific and haploid expressed. This study suggests its expression (diploid) by the epididymal epithelium. Quantitatively, Spam1 in epididymal and caudal sperm is affected differently by the translocations: there was no significant difference of the epididymal protein in normal and the Rb-bearing mice; but for caudal sperm the amounts were 70 and 100% of normal, for Rb(6.16) and Rb(6.15), respectively. These are higher than the previously reported 43 and 52% for testicular Spam1.
520 $a From these findings, it is proposed that sperm storage in the epididymis increases the quantity of Spam1 on sperm as well as its catalytic efficiency. The modulating effect of sperm aging on the dysfunction of Rb-bearing sperm may thus be explained by an increase in quantity and activity of Spam1 following prolonged sperm storage in the male.
590 $a School code: 0060.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biophysics, General. $3 1019105
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710 2 0 $a University of Delaware. $3 1017826
773 0 $t Dissertation Abstracts International $g 60-02B.
790 1 0 $a DeLeon, Patricia A., $e advisor
790 $a 0060
791 $a Ph.D.
792 $a 1999
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