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Genetic characterization of the medi...

Stevens, Jennitte LeAnn.

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Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function./
作者:	Stevens, Jennitte LeAnn.
面頁冊數:	107 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-07, Section: B, page: 3164.
Contained By:	Dissertation Abstracts International63-07B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3059561
ISBN:	0493752234

Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function.
Stevens, Jennitte LeAnn.

Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function. - 107 p.

Source: Dissertation Abstracts International, Volume: 63-07, Section: B, page: 3164.

Thesis (Ph.D.)--University of California, Los Angeles, 2002.

The Mediator complex is an important RNA polymerase II co-factor required for the expression of most eukaryotic protein-coding genes. It is believed to function by, integrating information from gene specific transcription factors to the general transcription machinery. Mediator function has been extensively characterized in S. cerevisiae, and more recent identification of Mediator complexes in metazoans indicates that Mediator is a universal co-factor of global transcription in all eukaryotes. There are several novel subunits in the Mediator complexes of metazoans that are not apparently found in yeast. Some of these are thought to be specific for the complex developmental and signal transduction programs required in higher organisms. Sur2, one such novel Mediator component, was identified in the human Mediator through its interaction with Adenovirus EIA conserved region 3 (CR3), a potent transcriptional transactivator of early adenoviral genes. Sur2 also appears to be a target of conserved RAS-RAF-Mitogen Activated Protein Kinase (MAPK) signal transduction pathways, as Sur2 mutants suppress an activated RAS phenotype in C. elegans vulva development.

ISBN: 0493752234Subjects--Topical Terms:

1017719
Biology, Molecular.

Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function.
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245 1 0 $a Genetic characterization of the mediator subunit Sur2 provides insight to metazoan mediator function.
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500 $a Source: Dissertation Abstracts International, Volume: 63-07, Section: B, page: 3164.
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502 $a Thesis (Ph.D.)--University of California, Los Angeles, 2002.
520 $a The Mediator complex is an important RNA polymerase II co-factor required for the expression of most eukaryotic protein-coding genes. It is believed to function by, integrating information from gene specific transcription factors to the general transcription machinery. Mediator function has been extensively characterized in S. cerevisiae, and more recent identification of Mediator complexes in metazoans indicates that Mediator is a universal co-factor of global transcription in all eukaryotes. There are several novel subunits in the Mediator complexes of metazoans that are not apparently found in yeast. Some of these are thought to be specific for the complex developmental and signal transduction programs required in higher organisms. Sur2, one such novel Mediator component, was identified in the human Mediator through its interaction with Adenovirus EIA conserved region 3 (CR3), a potent transcriptional transactivator of early adenoviral genes. Sur2 also appears to be a target of conserved RAS-RAF-Mitogen Activated Protein Kinase (MAPK) signal transduction pathways, as Sur2 mutants suppress an activated RAS phenotype in C. elegans vulva development.
520 $a To identify the specific role of Sur2 in MAPK signal transduction pathways, EIA-CR3 transactivation, mammalian development, and as a mammalian Mediator component, we generated a knockout in murine embryonic stem (ES) cells and in mice. We find that Sur2 is required for mammalian development, as sur2-/- embryos die during midgestation, suggesting that this Mediator subunit is needed for specific signal transduction pathways required during organogenesis, but not for Mediator function in general. Secondly, we find that Sur2 forms a subcomplex of the Mediator, with two other metazoan subunits, TRAP/Med 100 and TRAP/Med 95. This suggests that like the yeast Mediator, mammalian Mediator complexes are also modular in nature. Finally, analysis of activator function in the sur2-/- ES cells show specific defects in activation by 1A-CR3 and Elk-1, a MAPK-regulated, ETS-transcription factor, but not by other transcription factors tested. These results imply that specific activation domains stimulate transcription by binding to distinct Mediator subunits. Activation by E1A and Elk-1 requires recruitment of Mediator to a promoter through interaction with the Sur2 subunit.
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