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Whole-genome shotgun optical mapping...

Qi, Rong.

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Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping./
作者:	Qi, Rong.
面頁冊數:	200 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4390.
Contained By:	Dissertation Abstracts International60-09B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9945317
ISBN:	0599472979

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.
Qi, Rong.

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping. - 200 p.

Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4390.

Thesis (Ph.D.)--New York University, 1999.

Optical Mapping is a proven means for the rapid construction of accurate, ordered restriction maps from ensembles of individual surface-mounted DNA molecules. Whole genome shotgun Optical Mapping was used to analyze the genome of Deinococcus radiodurans, Trypanosoma brucei and Homo sapiens. A Nhe I map of the Deinococcus radiodurans genome (3.1 Mb) with a resolution of 29 kb was generated by both manual map assembly and computational means. A second chromosome (415 kb) was also discovered using Optical Mapping. A new approach was developed to anchor any sequences to optical maps aided by Southern blotting analysis. A preliminary Xba I map, with an average restriction fragment size of 17.7 kb, was constructed for the unicellular parasite, Trypanosoma brucei, which causes sleeping sickness in humans. The contigs consisted of 10 maps and spanned 12.6 Mb. A composite map was aligned with a sequenced BAC clone derived from chromosome II. One region containing putative Ingi repeats was also detected. Whole genome optical maps have significantly facilitated the sequence assembly, gap closure and verification efforts for the large-scale sequencing of Deinococcus radiodurans and Trypanosoma brucei genomes. A pilot project for construction of a high-resolution reference map of the entire human genome was carried out. Preliminary data showed good correspondence with a statistical model of map construction developed by Lander and Waterman. In order to achieve the goal of high throughput and high reliability for Optical Mapping, a system using a cross-linked acrylamide overlay was developed. We have defined conditions that reproducibly resulted in excellent digestion efficiencies of 75%--80% and the routine retention of restriction fragments as small as 1 kb. Patterns of a 4-cutter enzyme, HinP1 I, were also evaluated using the overlay system. The preliminary results will provide the foundation for the development of an ultra-high resolution Optical Mapping system.

ISBN: 0599472979Subjects--Topical Terms:

1017730
Biology, Genetics.

Whole-genome shotgun optical mapping of the Deinococcus radiodurans and Trypanosoma brucei genomes; and the development of a new surface system for optical mapping.
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