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Identification and characterization ...

Agostini, Heidi Joyce.

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Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans./
Author:	Agostini, Heidi Joyce.
Description:	159 p.
Notes:	Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3562.
Contained By:	Dissertation Abstracts International57-06B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9632629

Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans.
Agostini, Heidi Joyce.

Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans. - 159 p.

Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3562.

Thesis (Ph.D.)--Uniformed Services University of the Health Sciences, 1996.

Gram positive bacteria of the genus Deinococcus possess extraordinary resistance to the lethal and mutagenic effects of most agents that damage DNA, including ultraviolet (UV) radiation and the genotoxin mitomycin C (MMC). The extremely efficient ability to repair DNA damage induced by UV and MMC has been attributed, through complementation analysis, to two putative novel repair endonucleases (UV endonuclease-Subjects--Topical Terms:

1017719
Biology, Molecular.

Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans.
LDR:04091nmm 2200313 4500 001 1861991
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008 130614s1996 eng d
035 $a (UnM)AAI9632629
035 $a AAI9632629
040 $a UnM $c UnM
100 1 $a Agostini, Heidi Joyce. $3 1949566
245 1 0 $a Identification and characterization of UVRA, a DNA repair gene of Deinococcus radiodurans.
300 $a 159 p.
500 $a Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3562.
500 $a Director: Kenneth Minton.
502 $a Thesis (Ph.D.)--Uniformed Services University of the Health Sciences, 1996.
520 $a Gram positive bacteria of the genus Deinococcus possess extraordinary resistance to the lethal and mutagenic effects of most agents that damage DNA, including ultraviolet (UV) radiation and the genotoxin mitomycin C (MMC). The extremely efficient ability to repair DNA damage induced by UV and MMC has been attributed, through complementation analysis, to two putative novel repair endonucleases (UV endonuclease- $\ alpha $ and UV endonuclease- $\ beta) $ that incise deinococcal chromosomal DNA at or near sites of DNA damage, thereby initiating enzymatic excision of the DNA lesions. These repair endonucleases are encoded by the mtcA, mtcB (endonuclease- $\ alpha) $ and uvsC, uvsD, and uvsE (endonuclease- $\ beta) $ genes.
520 $a The gene encoded by mtcA and mtcB was found by DNA sequence analysis to be a single gene encoding a protein identified as the homolog of E. coli UvrA. UvrA is one protein of the UvrABC exonuclease complex in E. coli that recognizes thymine dimers and other bulky damage on nucleotide strands after UV induced DNA damage or other bulky DNA damage, such as MMC adducts. The nucleotide sequence of the uvrA homolog in D. radiodurans is over 60% homologous with E. coli and M. luteus uvrA nucleotide sequences. The UvrA homolog in D. radiodurans, like E. coli UvrA, contains amino acid sequence for two ATP binding sites as well as two zinc binding domains and one helix-turn-helix region. Several open reading frames adjacent to and divergently transcribed from uvrA (mtcA, mtcB), were found in the location in which the single-stranded binding protein (ssb) is normally located. The uvrA operator region of D. radiodurans was found to be nearly identical with the E. coli uvrA operator region; however, the lexA repressor binding motif was not fully conserved. E. coli, M. luteus, S. marcescans, B. abortus and now D. radiodurans have homologous genes encoding uvrA which indicate that this gene is highly conserved among Gram positive as well as Gram negative bacteria. The presence of a D. radiodurans gene homologous to uvrA of E. coli suggests the existence of an enzyme complex similar to UvrABC exonuclease encoded by uvrA, uvrB, and uvrC genes of E. coli. It is unclear at the time whether Deinococcus radiodurans possess a uvrB and a uvrC as seen in E. coli.
520 $a In addition, complementation studies were performed utilizing E. coli uvrA in the uvrA mutant strain 302. The strain 302 $\ Omega $2 pS11::uvrA yielded wild-type cell survival following treatments of MTC and 4-nitro-quinoline-1-oxide. The mutant strain 302 was incapable of repairing DNA damage caused by these chemicals. These studies indicate that E. coli uvrA can functionally replace D. radiodurans uvrA in repairing these forms of damage.
520 $a Finally, a 1,000 bp fragment including the 5 $\ sp\prime $ termini of the uvrA plus a short upstream segment (262 bp) was found to restore ionizing radiation resistance to the ionizing radiation mutant, IRS18. This finding suggests that the function of D. radiodurans uvrA may be more complex than E. coli uvrA, and that D. radiodurans uvrA may potentially play a role in ionizing radiation resistance.
590 $a School code: 0888.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Health Sciences, Pathology. $3 1017854
690 $a 0307
690 $a 0410
690 $a 0571
710 2 0 $a Uniformed Services University of the Health Sciences. $3 1916055
773 0 $t Dissertation Abstracts International $g 57-06B.
790 1 0 $a Minton, Kenneth, $e advisor
790 $a 0888
791 $a Ph.D.
792 $a 1996
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9632629