東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Characterization of ionizing radiati...

Udupa, Kumaraswamy S.

FindBook

Google Book

Amazon

博客來

Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1./
作者:	Udupa, Kumaraswamy S.
面頁冊數:	95 p.
附註:	Source: Dissertation Abstracts International, Volume: 57-05, Section: B, page: 3028.
Contained By:	Dissertation Abstracts International57-05B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9628321

Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1.
Udupa, Kumaraswamy S.

Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1. - 95 p.

Source: Dissertation Abstracts International, Volume: 57-05, Section: B, page: 3028.

Thesis (Ph.D.)--Louisiana State University and Agricultural & Mechanical College, 1996.

Deinococcus radiodurans is extremely resistant to ionizing radiation and can withstand 5 kGy Subjects--Topical Terms:

1017734
Biology, Microbiology.

Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1.
LDR:03210nmm 2200313 4500 001 1861990
005 20041215074545.5
008 130614s1996 eng d
035 $a (UnM)AAI9628321
035 $a AAI9628321
040 $a UnM $c UnM
100 1 $a Udupa, Kumaraswamy S. $3 1949565
245 1 0 $a Characterization of ionizing radiation sensitive (IRS) mutants of Deinococcus radiodurans R1.
300 $a 95 p.
500 $a Source: Dissertation Abstracts International, Volume: 57-05, Section: B, page: 3028.
500 $a Director: John R. Battista.
502 $a Thesis (Ph.D.)--Louisiana State University and Agricultural & Mechanical College, 1996.
520 $a Deinococcus radiodurans is extremely resistant to ionizing radiation and can withstand 5 kGy $\ gamma $ radiation without loss of viability or evidence of mutation. Available evidence argues that an efficient DNA repair mechanism is responsible for the resistance these organisms possess to ionizing radiation. The enzymes which coordinate DNA repair in D. radiodurans are poorly characterized.
520 $a Two new loci have been detected in D. radiodurans, namely, irrB and irrI which provide resistance. Mutational inactivation of either locus resulted in a partial loss of resistance to ionizing radiation. The extent of sensitivity was locus specific and differentially affected by inactivation of the uvrA gene product. An irrB uvrA double mutant was more sensitive to ionizing radiation than was an irrB mutant. In contrast, an irrI uvrA double mutant and irrI single mutant were equally sensitive to ionizing radiation. irrB and irrI mutations also reduced resistance to UV radiation in D. radiodurans which was more pronounced in the presence of an $u vrA\sp+ $ background. Subclones derived from each gene were generated and the loci of each gene were mapped relative to each other. The irrB and irrI genes were separated by approximately 20 kb of intervening sequence in which the uvrA and pol genes are located.
520 $a A mutation which inactivates the irrB gene does not impair repair of transforming DNA which has been damaged and contains thymine glycol or 8-hydroxyguanosine lesions. Examination of irrB cell extracts for novel DNA binding proteins revealed little information. irrB strains do produce a 23 kD novel protein (IrrB*) in large amounts but IrrB* did not bind to either ssDNA or dsDNA in the presence of 50 mM NaCl.
520 $a A mutation which impairs the function of the irrI gene product did not affect its potential to reactivate 8-hydroxyguanosine containing transforming DNA, but it failed to reactivate thymine glycol containing transforming DNA. In the absence of the irrI gene product D. radiodurans was extremely sensitive to UV radiation and its DNA underwent extensive degradation. The irrI gene product appears to regulate endonuclease $\ alpha $ mediated DNA degradation which occurs following exposure to UV radiation.
590 $a School code: 0107.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0369
690 $a 0307
710 2 0 $a Louisiana State University and Agricultural & Mechanical College. $3 783779
773 0 $t Dissertation Abstracts International $g 57-05B.
790 1 0 $a Battista, John R., $e advisor
790 $a 0107
791 $a Ph.D.
792 $a 1996
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9628321