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Below-ground biology of Botrychium p...

Camacho, Francisco Joaquin.

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Below-ground biology of Botrychium pumicola (Ophioglossaceae).

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Below-ground biology of Botrychium pumicola (Ophioglossaceae)./
作者:	Camacho, Francisco Joaquin.
面頁冊數:	132 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-06, Section: B, page: 2450.
Contained By:	Dissertation Abstracts International60-06B.
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9933184
ISBN:	0599337435

Below-ground biology of Botrychium pumicola (Ophioglossaceae).
Camacho, Francisco Joaquin.

Below-ground biology of Botrychium pumicola (Ophioglossaceae). - 132 p.

Source: Dissertation Abstracts International, Volume: 60-06, Section: B, page: 2450.

Thesis (Ph.D.)--Oregon State University, 1999.

Botrychium pumicola Coville is a rare fern with extant populations in Klamath, Lake and Deschutes counties, of central Oregon. It grows on subalpine pumice ridges and lower montane lodgepole pine forest openings on pumice-rich soils. The goal of this research was to better understand the below ground biology of B. pumicola. Detailed examination of the subterranean structures of B. pumicola revealed sporophytic gemmae attached to the stem. Developing gemmae are a non-photosynthesizing stage in the life cycle of this plant and are presumed to depend on mycorrhzial fungi for their nutrition. Population genetic analysis of B. pumicola using inter-simple sequence repeats (ISSR) suggests that the gemmae do not disperse far from the parent plant. Examination of the endophytic fungal structures in the roots of B. pumicola reveal arbuscular mycorrhziae and a high abundance of septate hyphae. To better characterize the root fungi, the internal transcribed spacer region of fungal ribosomal DNA (ITS) was amplified from root DNA by the polymerase chain reaction (PCR). The ITS amplicon was cloned and sequenced in order to characterize the different fungi in a root segment. Arbuscular mycorrhizal-like sequences were of low abundance in these clone libraries, whereas the dominant fungal group were ascomycetes. The most frequent fungi in these clone libraries are two commonly isolated sterile ascomycetes.

ISBN: 0599337435Subjects--Topical Terms:

1017825
Biology, Botany.

Below-ground biology of Botrychium pumicola (Ophioglossaceae).
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520 $a Botrychium pumicola Coville is a rare fern with extant populations in Klamath, Lake and Deschutes counties, of central Oregon. It grows on subalpine pumice ridges and lower montane lodgepole pine forest openings on pumice-rich soils. The goal of this research was to better understand the below ground biology of B. pumicola. Detailed examination of the subterranean structures of B. pumicola revealed sporophytic gemmae attached to the stem. Developing gemmae are a non-photosynthesizing stage in the life cycle of this plant and are presumed to depend on mycorrhzial fungi for their nutrition. Population genetic analysis of B. pumicola using inter-simple sequence repeats (ISSR) suggests that the gemmae do not disperse far from the parent plant. Examination of the endophytic fungal structures in the roots of B. pumicola reveal arbuscular mycorrhziae and a high abundance of septate hyphae. To better characterize the root fungi, the internal transcribed spacer region of fungal ribosomal DNA (ITS) was amplified from root DNA by the polymerase chain reaction (PCR). The ITS amplicon was cloned and sequenced in order to characterize the different fungi in a root segment. Arbuscular mycorrhizal-like sequences were of low abundance in these clone libraries, whereas the dominant fungal group were ascomycetes. The most frequent fungi in these clone libraries are two commonly isolated sterile ascomycetes.
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