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Methods to optimize immunoassays for...

Young, Denise.

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Methods to optimize immunoassays for the detection of foodborne pathogens.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Methods to optimize immunoassays for the detection of foodborne pathogens./
Author:	Young, Denise.
Description:	121 p.
Notes:	Source: Masters Abstracts International, Volume: 42-01, page: 0135.
Contained By:	Masters Abstracts International42-01.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ80209
ISBN:	0612802094

Methods to optimize immunoassays for the detection of foodborne pathogens.
Young, Denise.

Methods to optimize immunoassays for the detection of foodborne pathogens. - 121 p.

Source: Masters Abstracts International, Volume: 42-01, page: 0135.

Thesis (M.Sc.)--University of Guelph (Canada), 2003.

Immunoassays are routinely used for the rapid detection of foodborne pathogens and spoilage/indicator bacteria. Antibodies used for detection need to have high specificity and affinity towards live bacterial cells. Assays combining the use of immunomagnetic separation (IMS) and lux and gfp gene labels were developed for the determination of antibody affinity. Antibody affinities were determined and compared to enzyme-linked immunosorbent assay (ELISA) sensitivities in varying pH, sodium chloride, and temperature conditions. The bioluminescence assay was sensitive for determining affinity. For assays using bioluminescent S. Enteritidis and E. coli O157:H7, increases in sodium chloride levels resulted in higher affinities, however changes in temperatures had no significant effect. A higher affinity and ELISA sensitivity was obtained for anti-E. coli O157:H7 antibodies at pH 3 compared to pH 5 and 7. No relationship was found between affinity and ELISA sensitivities using various sodium chloride and temperature conditions.

ISBN: 0612802094Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Methods to optimize immunoassays for the detection of foodborne pathogens.
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245 1 0 $a Methods to optimize immunoassays for the detection of foodborne pathogens.
300 $a 121 p.
500 $a Source: Masters Abstracts International, Volume: 42-01, page: 0135.
500 $a Adviser: M. W. Griffiths.
502 $a Thesis (M.Sc.)--University of Guelph (Canada), 2003.
520 $a Immunoassays are routinely used for the rapid detection of foodborne pathogens and spoilage/indicator bacteria. Antibodies used for detection need to have high specificity and affinity towards live bacterial cells. Assays combining the use of immunomagnetic separation (IMS) and lux and gfp gene labels were developed for the determination of antibody affinity. Antibody affinities were determined and compared to enzyme-linked immunosorbent assay (ELISA) sensitivities in varying pH, sodium chloride, and temperature conditions. The bioluminescence assay was sensitive for determining affinity. For assays using bioluminescent S. Enteritidis and E. coli O157:H7, increases in sodium chloride levels resulted in higher affinities, however changes in temperatures had no significant effect. A higher affinity and ELISA sensitivity was obtained for anti-E. coli O157:H7 antibodies at pH 3 compared to pH 5 and 7. No relationship was found between affinity and ELISA sensitivities using various sodium chloride and temperature conditions.
590 $a School code: 0081.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
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