語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Enhancement of DNA immunization agai...
~
Balasubramanian, Sowmya.
FindBook
Google Book
Amazon
博客來
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules./
作者:
Balasubramanian, Sowmya.
面頁冊數:
132 p.
附註:
Source: Masters Abstracts International, Volume: 39-04, page: 1087.
Contained By:
Masters Abstracts International39-04.
標題:
Biology, Molecular. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ57082
ISBN:
0612570827
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules.
Balasubramanian, Sowmya.
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules.
- 132 p.
Source: Masters Abstracts International, Volume: 39-04, page: 1087.
Thesis (M.Sc.)--University of Ottawa (Canada), 2000.
The use of plasmid DNA encoding antigens has given rise to a novel class of vaccines, that may overcome many of the disadvantages associated with classical antigen-based vaccines. This thesis examines specific methods of optimizing immune responses directed against the hepatitis B virus surface antigen (HBsAg) encoded by a plasmid expression system in BALB/c mice. Interaction of B7.1 and B7.2 with their receptor CD28/CTLA-4 molecules on T cells is known to facilitate progression of the T cell through the cycle of immune activation. We have determined whether coexpression of costimulatory molecules, B7.1 or B7.2, along with HBsAg from a DNA vaccine, administered as separate plasmids (codelivery) or encoded within the same expression vector (colinear expression), can augment HBsAg-specific humoral and cell-mediated responses. Intramuscular (IM) coexpression of B7.1 or B7.2 along with the DNA vaccine significantly enhanced cytotoxic T lymphocyte (CTL) responses whereas coexpression of B7.2 alone enhanced CTL responses with intradermal (ID) administration. However, there was no concomitant increase in humoral responses with coexpression by either route of administration. In contrast colinear expression of B7.1 or B7.2 significantly enhanced humoral as well as CTL responses with both IM and ID routes of administration. Furthermore, the Th2 bias that is seen with ID administration is skewed towards a Th1 response with B7 coexpression. The kinetics of plasmid DNA distribution in various anatomical compartments were also studied in order to address the differences in immune responses noted with IM and ID routes of administration. Collectively the results suggest that B7 coexpression can augment or alter responses to DNA vaccines and might prove effective for immunization in humans.
ISBN: 0612570827Subjects--Topical Terms:
1017719
Biology, Molecular.
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules.
LDR
:02702nmm 2200277 4500
001
1855814
005
20040629073633.5
008
130614s2000 eng d
020
$a
0612570827
035
$a
(UnM)AAIMQ57082
035
$a
AAIMQ57082
040
$a
UnM
$c
UnM
100
1
$a
Balasubramanian, Sowmya.
$3
1943614
245
1 0
$a
Enhancement of DNA immunization against hepatitis B following coexpression of costimulatory molecules.
300
$a
132 p.
500
$a
Source: Masters Abstracts International, Volume: 39-04, page: 1087.
500
$a
Adviser: Heather L. Davis.
502
$a
Thesis (M.Sc.)--University of Ottawa (Canada), 2000.
520
$a
The use of plasmid DNA encoding antigens has given rise to a novel class of vaccines, that may overcome many of the disadvantages associated with classical antigen-based vaccines. This thesis examines specific methods of optimizing immune responses directed against the hepatitis B virus surface antigen (HBsAg) encoded by a plasmid expression system in BALB/c mice. Interaction of B7.1 and B7.2 with their receptor CD28/CTLA-4 molecules on T cells is known to facilitate progression of the T cell through the cycle of immune activation. We have determined whether coexpression of costimulatory molecules, B7.1 or B7.2, along with HBsAg from a DNA vaccine, administered as separate plasmids (codelivery) or encoded within the same expression vector (colinear expression), can augment HBsAg-specific humoral and cell-mediated responses. Intramuscular (IM) coexpression of B7.1 or B7.2 along with the DNA vaccine significantly enhanced cytotoxic T lymphocyte (CTL) responses whereas coexpression of B7.2 alone enhanced CTL responses with intradermal (ID) administration. However, there was no concomitant increase in humoral responses with coexpression by either route of administration. In contrast colinear expression of B7.1 or B7.2 significantly enhanced humoral as well as CTL responses with both IM and ID routes of administration. Furthermore, the Th2 bias that is seen with ID administration is skewed towards a Th1 response with B7 coexpression. The kinetics of plasmid DNA distribution in various anatomical compartments were also studied in order to address the differences in immune responses noted with IM and ID routes of administration. Collectively the results suggest that B7 coexpression can augment or alter responses to DNA vaccines and might prove effective for immunization in humans.
590
$a
School code: 0918.
650
4
$a
Biology, Molecular.
$3
1017719
650
4
$a
Health Sciences, Immunology.
$3
1017716
690
$a
0307
690
$a
0982
710
2 0
$a
University of Ottawa (Canada).
$3
1017488
773
0
$t
Masters Abstracts International
$g
39-04.
790
1 0
$a
Davis, Heather L.,
$e
advisor
790
$a
0918
791
$a
M.Sc.
792
$a
2000
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ57082
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9174514
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入