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Delivery of polynucleotides and olig...

Babiuk, Shawn.

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Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines./
作者:	Babiuk, Shawn.
面頁冊數:	212 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4271.
Contained By:	Dissertation Abstracts International64-09B.
標題:	Health Sciences, Immunology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ83545
ISBN:	0612835456

Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines.
Babiuk, Shawn.

Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines. - 212 p.

Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4271.

Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2003.

The development of DNA based vaccines and immunostimulatory CpG oligonucleotides (ODNs) as adjuvants offers possibilities for developing new vaccines.

ISBN: 0612835456Subjects--Topical Terms:

1017716
Health Sciences, Immunology.

Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines.
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245 1 0 $a Delivery of polynucleotides and oligonucleotides for improving immune responses to vaccines.
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500 $a Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4271.
500 $a Adviser: Marianna Foldvari.
502 $a Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2003.
520 $a The development of DNA based vaccines and immunostimulatory CpG oligonucleotides (ODNs) as adjuvants offers possibilities for developing new vaccines.
520 $a The development of non-invasive methods for the delivery of vaccines through the skin will greatly improve the safety and the administration of human and veterinary vaccines for different species. This study examined the efficiency of topical delivery of plasmids by assessing the localization of gene expression using luciferase as a reporter gene and induction of immune responses using a plasmid encoding for the bovine herpesvirus type-1 glycoprotein D (pgD). Topical administration of plasmids in a lipid based delivery system (biphasic lipid vesicles [Biphasix™]) resulted in gene expression in the draining lymph nodes, whereas with intradermal injection, antigen expression was found in the skin. Following administration of plasmid by gene gun, antigen expression was observed in both the skin and the draining lymph nodes. Transcutaneous immunization with pgD formulated in biphasic lipid vesicles elicited gD-specific antibody responses and a Th2 type cellular response. In contrast, immunization by the intradermal route resulted in the stimulation of a Th1 type response. These findings have implications for both vaccine design and tailoring of specific immune responses.
520 $a Electroporation has been shown to increase the potency of DNA vaccines that have demonstrated significant potential in mice. However, there is a need to develop non-invasive or minimally invasive vaccination methods. In pigs, <italic>in vivo</italic> gene expression was assessed to compare intradermal needle injection to a needle-free dermal BioJect™ as a means of delivery of plasmids. Each administration method was further tested with and without surface electroporation. Experiments with plasmid DNA encoding luciferase demonstrated that needle-free administration results in higher expression levels than needle injection. Electroporation strongly enhanced gene expression for both intradermal delivery methods and immune responses to a DNA vaccine encoding hepatitis B surface antigen. Needle-free plasmid injection in combination with electroporation led to a more rapid induction of immune responses compared to other methods of plasmid administration. Priming and boosting with the DNA vaccine heightened the effectiveness of a subsequent protein boost, and electroporation after DNA injection augmented those benefits. (Abstract shortened by UMI.)
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791 $a Ph.D.
792 $a 2003
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