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Novel formulation: Development of or...

Tantituvanont, Angkana.

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Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation./
Author:	Tantituvanont, Angkana.
Description:	331 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3216.
Contained By:	Dissertation Abstracts International64-07B.
Subject:	Health Sciences, Pharmacy. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098445

Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation.
Tantituvanont, Angkana.

Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation. - 331 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3216.

Thesis (Ph.D.)--Oregon State University, 2003.

This dissertation describes two different projects. The first is the development of an oral DNA vaccine delivery system for fish. A novel oral DNA vaccine delivery system was developed for Rainbow Trout by combining non-viral vectors (polycationic liposomes or polycationic polymer) to facilitate the DNA vaccine's uptake by cell membranes along with enteric-coated protection of the DNA embedded in microparticles to prevent DNA degradation in the gastrointestinal tract. Spray drying and spray coating bead techniques were employed in the preparation of the DNA vaccine microparticles. The spray drying technique allowed production of spherical shape enteric-coated microparticles with a particle size range of 0.18 to 20 μm. Larger particle sizes of 40–50 mesh were obtained from the spray-coated bead technique. The resultant DNA vaccine microparticles were granulated with regular fish feed and given to fish to investigate the efficacy of the delivery system in providing protection against IHNV, and to demonstrate the ease of administration in fish. An <italic> in vivo</italic> fish trial experiment showed improvement in fish survival rate when fish were immunized with larger particle size DNA vaccine microparticles. Further research to find effective vector carriers for the DNA vaccine delivery system and to seek modifications of the delivery system that will still prevent the denaturation of plasmid DNA that will also facilitate membrane uptake of the DNA vaccine is needed in order to develop a safe, effective, and commercially viable vaccine to control the outbreak of IHNV.Subjects--Topical Terms:

1017737
Health Sciences, Pharmacy.

Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation.
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100 1 $a Tantituvanont, Angkana. $3 1943578
245 1 0 $a Novel formulation: Development of oral microparticulate non-viral DNA vaccine delivery system against infectious hematopoietic necrosis virus (IHNV) in rainbow trout, statistical design in matrix tablets formulation.
300 $a 331 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3216.
500 $a Adviser: John Mark Christensen.
502 $a Thesis (Ph.D.)--Oregon State University, 2003.
520 $a This dissertation describes two different projects. The first is the development of an oral DNA vaccine delivery system for fish. A novel oral DNA vaccine delivery system was developed for Rainbow Trout by combining non-viral vectors (polycationic liposomes or polycationic polymer) to facilitate the DNA vaccine's uptake by cell membranes along with enteric-coated protection of the DNA embedded in microparticles to prevent DNA degradation in the gastrointestinal tract. Spray drying and spray coating bead techniques were employed in the preparation of the DNA vaccine microparticles. The spray drying technique allowed production of spherical shape enteric-coated microparticles with a particle size range of 0.18 to 20 μm. Larger particle sizes of 40–50 mesh were obtained from the spray-coated bead technique. The resultant DNA vaccine microparticles were granulated with regular fish feed and given to fish to investigate the efficacy of the delivery system in providing protection against IHNV, and to demonstrate the ease of administration in fish. An <italic> in vivo</italic> fish trial experiment showed improvement in fish survival rate when fish were immunized with larger particle size DNA vaccine microparticles. Further research to find effective vector carriers for the DNA vaccine delivery system and to seek modifications of the delivery system that will still prevent the denaturation of plasmid DNA that will also facilitate membrane uptake of the DNA vaccine is needed in order to develop a safe, effective, and commercially viable vaccine to control the outbreak of IHNV.
520 $a The second project of the dissertation is prediction of <italic>in vitro </italic> drug release profiles from a novel matrix tablet spray-coated with a barrier membrane using mathematical and statistical models. Tablets were prepared by direct compression followed by spray coating. The relationship of the amount of hydrophilic materials in the core tablets and barrier thickness on drug release mechanism was investigated using factorial design and regression analysis. Drug release characteristics were influenced and can be controlled by modifying the amount of hydrophilic materials in the core tablet and the barrier thickness. Mathematical equation generated from regression analysis of n-value, lag time, and percent drug release as a function of the amount of hydrophilic material and the amount of coating material applied can now be used as a tool for predicting and optimizing <italic>in vitro</italic> drug release from matrix tablets spray-coated with a barrier membrane.
590 $a School code: 0172.
650 4 $a Health Sciences, Pharmacy. $3 1017737
650 4 $a Agriculture, Animal Pathology. $3 1021764
650 4 $a Agriculture, Fisheries and Aquaculture. $3 1020913
690 $a 0572
690 $a 0476
690 $a 0792
710 2 0 $a Oregon State University. $3 625720
773 0 $t Dissertation Abstracts International $g 64-07B.
790 1 0 $a Christensen, John Mark, $e advisor
790 $a 0172
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098445