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Physiological and molecular response...

Nepomuceno, Alexandre Lima.

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Physiological and molecular responses of plants to water deficit.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Physiological and molecular responses of plants to water deficit./
作者:	Nepomuceno, Alexandre Lima.
面頁冊數:	276 p.
附註:	Source: Dissertation Abstracts International, Volume: 59-06, Section: B, page: 2614.
Contained By:	Dissertation Abstracts International59-06B.
標題:	Biology, Plant Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9838315
ISBN:	0591919877

Physiological and molecular responses of plants to water deficit.
Nepomuceno, Alexandre Lima.

Physiological and molecular responses of plants to water deficit. - 276 p.

Source: Dissertation Abstracts International, Volume: 59-06, Section: B, page: 2614.

Thesis (Ph.D.)--University of Arkansas, 1998.

Tolerance to drought in plants results from networks of mechanisms working isolated or in combination. Genotypes that differ in tolerance to water-deficit stress should have qualitative and/or quantitative differences in gene expression. Therefore, to identify and isolate genes, which may differ among four cotton genotypes with diverse responses to water deprivation, Differential Display (DDRT-PCR) was used. These genotypes were first physiologically characterized in growth chamber and greenhouse experiments. Water and osmotic potential of roots and leaves, photosynthetic rate and relative water content were some of the physiological parameters used to measure plant responses during and after the water deficit applied. Cultivar Siokra L-23 and the wild type T-1521, two drought tolerant genotypes, showed a significant ability of osmotic adjust during water deficit. Photosynthetic rate and relative water content were maintained near control values in the two tolerant genotypes. However, cultivar CS 50 and Stoneville 506, showed significant sensitivity to the water deficit applied, confirming previous observations. These results guided subsequent work with DDRT-PCR using mRNA samples collected during these experiments. Fifty-two cDNA fragments differentially expressed during water deficit were identified using DDRT-PCR. The differentially displayed fragments were isolated from the polyacrylamide gels and cloned in pGEM-T vectors. After cloning they were sequenced using thermocycling dideoxynucleotide chain termination protocols. Search of gene banks revealed 17 clones with high homology to known genes, and 20 clones with low homology, while 15 clones had no homologous entries. Among the sequences that showed high homology was a heat shock protein that binds to calmodulin. This gene was expressed only in the water-deficit tolerant genotypes (Siokra L-23 and T-1521) during the stress. Another gene identified by homology was trehalose-6-phosphate synthase. This enzyme is part of the metabolic pathway for production of trehalose, a sugar known to osmoprotect cell membranes during desiccation. The differentially expressed gene sequences could have use in screening germplasm banks for similar genotypes, in plant physiology studies, and in molecular mapping of plant responses to water deficit.

ISBN: 0591919877Subjects--Topical Terms:

1017865
Biology, Plant Physiology.

Physiological and molecular responses of plants to water deficit.
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