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Taura syndrome of marine penaeid shr...
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Hasson, Kenneth Wolf.
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Taura syndrome of marine penaeid shrimp: Discovery of the viral agent and disease characterization studies.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Taura syndrome of marine penaeid shrimp: Discovery of the viral agent and disease characterization studies./
作者:
Hasson, Kenneth Wolf.
面頁冊數:
365 p.
附註:
Source: Dissertation Abstracts International, Volume: 59-05, Section: B, page: 1931.
Contained By:
Dissertation Abstracts International59-05B.
標題:
Agriculture, Fisheries and Aquaculture. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9831832
ISBN:
0591847779
Taura syndrome of marine penaeid shrimp: Discovery of the viral agent and disease characterization studies.
Hasson, Kenneth Wolf.
Taura syndrome of marine penaeid shrimp: Discovery of the viral agent and disease characterization studies.
- 365 p.
Source: Dissertation Abstracts International, Volume: 59-05, Section: B, page: 1931.
Thesis (Ph.D.)--The University of Arizona, 1998.
These studies were begun during the summer of 1994, W2 years after the recognition of Taura Syndrome (TS), a lethal disease of farm-raised Penaeus vannamei in Ecuador of then unknown etiology.
ISBN: 0591847779Subjects--Topical Terms:
1020913
Agriculture, Fisheries and Aquaculture.
Taura syndrome of marine penaeid shrimp: Discovery of the viral agent and disease characterization studies.
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Source: Dissertation Abstracts International, Volume: 59-05, Section: B, page: 1931.
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Director: Donald V. Lightner.
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Thesis (Ph.D.)--The University of Arizona, 1998.
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These studies were begun during the summer of 1994, W2 years after the recognition of Taura Syndrome (TS), a lethal disease of farm-raised Penaeus vannamei in Ecuador of then unknown etiology.
520
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Utilizing specific pathogen free (SPF) P. vannamei test shrimp, the combined results of four initial TS bioassays suggested that the disease had an infectious instead of a toxic etiology as originally reported.
520
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The hypothesis that TS was virus-caused prompted the application of a shrimp parvovirus (IHHNV) purification protocol, which resulted in the discovery of Taura Syndrome virus (TSV), a previously unrecognized virus that was isolated from TS diseased shrimp tissues.
520
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Three serial infectivity studies were performed in which purified cell-free extracts of TSV were injected into SPF P. vannamei test shrimp and the criteria of Rivers' postulates were fulfilled, establishing that TS has a viral etiology.
520
$a
In situ hybridization assays for the detection of TSV resulted in frequent false negative gene probe results. This problem was due to fixative-induced acid hydrolysis of the TSV RNA genome resulting from tissue fixation with Davidson's solution (pH 3.5-4). Development and use of a neutral fixative, R-F (RNA-Friendly) fixative, was shown to prevent this problem.
520
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The pathogenesis of TSV lesions was analyzed in experimentally infected, time course sampled SPF P. vannamei. The TSV disease cycle was found to consist of three clinically and histologically distinct overlapping phases; an W7 d acute phase, an W5 d transition phase and a long term cyclic chronic phase of at least 8 months duration.
520
$a
TSV susceptibility studies of two endemic North American (P. setiferus and P. aztecus) and one Asian penaeid species (P. chinensis) showed that P. aztecus and P. chinensis juveniles were susceptible, whereas, P. setiferus appeared refractory to TSV infection.
520
$a
The geographic range of TSV within the Americas was documented based on gene probe and histological findings of archived P. vannamei samples dating from 1992 to 1996. TSV was detectable within P. vannamei submitted from Ecuador when the disease was first recognized (1992) and has since spread into 12 other countries.
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The causes and effects of the international controversy of a viral versus a toxic etiology for TS are discussed and a solution offered to prevent similar disputes in the future.
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School code: 0009.
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http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9831832
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