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Metabolic pathway analysis and engin...

Carlson, Ross Peter.

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Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms./
Author:	Carlson, Ross Peter.
Description:	310 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3392.
Contained By:	Dissertation Abstracts International64-07B.
Subject:	Engineering, Chemical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098575

Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms.
Carlson, Ross Peter.

Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms. - 310 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3392.

Thesis (Ph.D.)--University of Minnesota, 2003.

All living organisms operate on the basis of a complex, highly branched and highly coupled biochemical network. These networks are capable of a wide variety of diverse chemistries like the fixing of CO<sub>2</sub> to produce organisms as large as trees or the catabolism of simple sugars to produce organisms as small as bacteria. Understanding how the networks are organized and how metabolites navigate the numerous pathways has significant implications on both the basic understanding of biological systems as well as the efficient conversion of substrate into commercially important chemicals like ethanol, hydrogen or antibiotics. The research described in this thesis entails the study of native metabolic networks with the goal of understanding their design principles and defining their capabilities. This is a fundamental step in understanding a system as complex as a biological organism. Predictions related to an <italic>Escherichia coli</italic> network were found to be in remarkable agreement with available published data. Having an improved understanding of the native system provides a more rational basis for introducing new chemistries or modifying the existing reaction network. In this thesis, biochemical networks are also analyzed for their theoretical ability to supply metabolic fluxes to foreign pathways. This information was used to guide the culturing, the analysis, and the genetic modification of recombinant <italic>Escherichia coli</italic> and <italic>Saccharomyces cerevisiae</italic> strains that had been engineered to produce the bioplastic polyhydroxyalkanoate (PHA). Predictions related to culturing conditions like the feasibility of anaerobic PHA production and predictions related to culture behavior like the co-production of PHA and ethanol were experimentally verified.Subjects--Topical Terms:

1018531
Engineering, Chemical.

Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms.
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035 $a (UnM)AAI3098575
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100 1 $a Carlson, Ross Peter. $3 1943074
245 1 0 $a Metabolic pathway analysis and engineering of prokaryotic and eukaryotic organisms.
300 $a 310 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3392.
500 $a Adviser: Friedrich Srienc.
502 $a Thesis (Ph.D.)--University of Minnesota, 2003.
520 $a All living organisms operate on the basis of a complex, highly branched and highly coupled biochemical network. These networks are capable of a wide variety of diverse chemistries like the fixing of CO<sub>2</sub> to produce organisms as large as trees or the catabolism of simple sugars to produce organisms as small as bacteria. Understanding how the networks are organized and how metabolites navigate the numerous pathways has significant implications on both the basic understanding of biological systems as well as the efficient conversion of substrate into commercially important chemicals like ethanol, hydrogen or antibiotics. The research described in this thesis entails the study of native metabolic networks with the goal of understanding their design principles and defining their capabilities. This is a fundamental step in understanding a system as complex as a biological organism. Predictions related to an <italic>Escherichia coli</italic> network were found to be in remarkable agreement with available published data. Having an improved understanding of the native system provides a more rational basis for introducing new chemistries or modifying the existing reaction network. In this thesis, biochemical networks are also analyzed for their theoretical ability to supply metabolic fluxes to foreign pathways. This information was used to guide the culturing, the analysis, and the genetic modification of recombinant <italic>Escherichia coli</italic> and <italic>Saccharomyces cerevisiae</italic> strains that had been engineered to produce the bioplastic polyhydroxyalkanoate (PHA). Predictions related to culturing conditions like the feasibility of anaerobic PHA production and predictions related to culture behavior like the co-production of PHA and ethanol were experimentally verified.
590 $a School code: 0130.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Chemistry, Biochemistry. $3 1017722
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710 2 0 $a University of Minnesota. $3 676231
773 0 $t Dissertation Abstracts International $g 64-07B.
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098575