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Genetic studies of RNA-mediated DNA ...

Liu, Yifan.

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Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila./
Author:	Liu, Yifan.
Description:	219 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3647.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3102284

Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila.
Liu, Yifan.

Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila. - 219 p.

Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3647.

Thesis (Ph.D.)--The University of Rochester, 2003.

Post-translational modification of histones is a highly conserved mechanism for modulating the dynamic structure of chromatin and is involved in transcription regulation. Histone H3 lysine 9 methylation is a hallmark of heterochromatin formation and gene silencing in a wide range of eukaryotes. In <italic>Tetrahymena </italic>, this modification and heterochromatin formation occur exclusively in the developing macronuclei, in association with the DNA sequences targeted for deletion. RNA-mediated gene silencing has recently been implicated in the initial targeting of H3 lysine 9 methylation and heterochromatin formation in <italic>S. pombe</italic>, as well as in the developmentally regulated DNA elimination in <italic>Tetrahymena</italic>.

Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila.
LDR:01998nmm 2200181 4500 001 1854463
005 20040524103735.5
008 130614s2003 eng d
035 $a (UnM)AAI3102284
035 $a AAI3102284
040 $a UnM $c UnM
100 1 $a Liu, Yifan. $3 1294825
245 1 0 $a Genetic studies of RNA-mediated DNA elimination in the developing macronuclei of Tetrahymena thermophila.
300 $a 219 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3647.
500 $a Supervisor: Martin A. Gorovsky.
502 $a Thesis (Ph.D.)--The University of Rochester, 2003.
520 $a Post-translational modification of histones is a highly conserved mechanism for modulating the dynamic structure of chromatin and is involved in transcription regulation. Histone H3 lysine 9 methylation is a hallmark of heterochromatin formation and gene silencing in a wide range of eukaryotes. In <italic>Tetrahymena </italic>, this modification and heterochromatin formation occur exclusively in the developing macronuclei, in association with the DNA sequences targeted for deletion. RNA-mediated gene silencing has recently been implicated in the initial targeting of H3 lysine 9 methylation and heterochromatin formation in <italic>S. pombe</italic>, as well as in the developmentally regulated DNA elimination in <italic>Tetrahymena</italic>.
520 $a Genetic studies were conducted to further understand the mechanism of DNA elimination in <italic>Tetrahymena</italic>. Mutating the lysine 9 residue in H3 prevents its methylation and disrupts DNA elimination, but does not affect the accumulation of small RNAs. Knocking out the <italic>TWI1</italic> gene, which is required for the accumulation of small RNAs and DNA elimination, also abolishes H3 lysine 9 methylation. This indicates that small RNA generation is upstream of H3 lysine 9 methylation, which is in turn required for DNA elimination in <italic>Tetrahymena</italic>
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3102284