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The role of the glutamate decarboxyl...

Park, Geunwoo.

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The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC).

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC)./
作者:	Park, Geunwoo.
面頁冊數:	165 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6107.
Contained By:	Dissertation Abstracts International65-12B.
標題:	Agriculture, Food Science and Technology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3156792
ISBN:	0496175017

The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC).
Park, Geunwoo.

The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC). - 165 p.

Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6107.

Thesis (Ph.D.)--University of Minnesota, 2005.

The glutamate decarboxylase (GAD)-mediated acid resistance is a mechanism that allows Escherichia coli to survive extreme acid conditions (pH ≤ 2.0). This unique characteristic was used as the basis of this thesis.

ISBN: 0496175017Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC).
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245 1 4 $a The role of the glutamate decarboxylase-mediated acid resistance on the environmental survival and the isolation of enterohemorrhagic Escherichia coli (EHEC).
300 $a 165 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6107.
500 $a Adviser: Francisco Diez-Gonzalez.
502 $a Thesis (Ph.D.)--University of Minnesota, 2005.
520 $a The glutamate decarboxylase (GAD)-mediated acid resistance is a mechanism that allows Escherichia coli to survive extreme acid conditions (pH ≤ 2.0). This unique characteristic was used as the basis of this thesis.
520 $a First, the use of glutamate-containing acid shocks in combination with plating on washed blood plates was investigated to detect enterohemorrhagic Escherichia coli (EHEC). When an acid shock treatment was used to test the ability of enteric bacteria cultures in resisting low pH (2.0--2.5), the survival rate of E. coli strains consistently increased in the presence of glutamate. This acid shock treatment was more effective in suppressing the growth of other enteric bacteria as compared to the use of potassium tellurite and cefixime. Further experiments using inoculated cattle fecal samples suggested that acid shocks allowed EHEC populations on the blood agar to improve its identification. When the EHEC count in fecal samples were below 2 log10 CFU/g, EHEC could not be identified by direct plating after acid shock, but a two step enrichment procedure allowed the detection of at least 1% of EHEC. These results suggested that if the initial population of EHEC was more than 100 CFU/g, the combined use of GAD-mediated acid resistance and enterohemolysin in EHEC might be an alternative way to improve the isolation of EHEC.
520 $a Second, the potential protective role of the GAD-mediated acid resistance for E. coli during acid decontamination of meat was assessed using a non-pathogenic strain and its gadAB mutant. The effect of several organic acids on E. coli survival was determined in pure cultures and in inoculated meat pieces. In vitro acid shock assays indicated that E. coli was drastically inactivated by fumaric acid even if was supplemented with 300 mM glutamate. When the meat pieces was inoculated with the E. coli strains, the survival rate of the wild-type strain was always greater than the gadAB mutant after acid treatment if the meat surfaces had free glutamate. However, no differences in viable cell reduction between these strains were determined when meat pieces were inoculated on intact inner skin of meat pieces. These results suggested that the ability of E. coli to resist to acid treatments is linked to glutamate levels on the meat surface.
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650 4 $a Biology, Microbiology. $3 1017734
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710 2 0 $a University of Minnesota. $3 676231
773 0 $t Dissertation Abstracts International $g 65-12B.
790 1 0 $a Diez-Gonzalez, Francisco, $e advisor
790 $a 0130
791 $a Ph.D.
792 $a 2005
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