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Determination of lactose by reversed...

Sexton, Danessa.

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Determination of lactose by reversed-phase high-performance liquid chromatography.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Determination of lactose by reversed-phase high-performance liquid chromatography./
作者:	Sexton, Danessa.
面頁冊數:	94 p.
附註:	Source: Masters Abstracts International, Volume: 42-05, page: 1713.
Contained By:	Masters Abstracts International42-05.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418655
ISBN:	0496234285

Determination of lactose by reversed-phase high-performance liquid chromatography.
Sexton, Danessa.

Determination of lactose by reversed-phase high-performance liquid chromatography. - 94 p.

Source: Masters Abstracts International, Volume: 42-05, page: 1713.

Thesis (M.S.)--East Tennessee State University, 2004.

Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300--360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.

ISBN: 0496234285Subjects--Topical Terms:

586156
Chemistry, Analytical.

Determination of lactose by reversed-phase high-performance liquid chromatography.
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520 $a Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300--360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
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