東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

An investigation of the application ...

Mann, Tina Louise.

FindBook

Google Book

Amazon

博客來

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors./
作者:	Mann, Tina Louise.
面頁冊數:	118 p.
附註:	Source: Masters Abstracts International, Volume: 42-03, page: 0940.
Contained By:	Masters Abstracts International42-03.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ84154
ISBN:	0612841545

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
Mann, Tina Louise.

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors. - 118 p.

Source: Masters Abstracts International, Volume: 42-03, page: 0940.

Thesis (M.Sc.)--University of Toronto (Canada), 2003.

Ultrasound exposure was examined as a method to cleave genomic DNA in a rapid manner. High frequency ultrasound of 20 kHz was applied to DNA solutions under a variety of experimental conditions. A Yates Pattern Analysis determined that ionic strength and the duration of sonication were the most significant factors impacting fragmentation patterns. A salt concentration of 1 M caused genomic DNA to resist fragmentation and fragment sizes were centred near 500 base pairs and larger. Decreasing the ionic strength to 0.l M NaCl or less resulted in the formation of fragment sizes close to 100 base pairs. Manipulation of these factors allowed for some control of fragmentation patterns. Hyperchromicity experiments, studies considering the influence of various gases, and simulations to determine the probability of destroying a target sequence were also done. Experiments suggested that ultrasound can be used as a rapid method to generate DNA fragments.

ISBN: 0612841545Subjects--Topical Terms:

586156
Chemistry, Analytical.

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
LDR:01878nmm 2200277 4500 001 1847790
005 20051108095758.5
008 130614s2003 eng d
020 $a 0612841545
035 $a (UnM)AAIMQ84154
035 $a AAIMQ84154
040 $a UnM $c UnM
100 1 $a Mann, Tina Louise. $3 1935821
245 1 3 $a An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
300 $a 118 p.
500 $a Source: Masters Abstracts International, Volume: 42-03, page: 0940.
500 $a Adviser: Ulrich Krull.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2003.
520 $a Ultrasound exposure was examined as a method to cleave genomic DNA in a rapid manner. High frequency ultrasound of 20 kHz was applied to DNA solutions under a variety of experimental conditions. A Yates Pattern Analysis determined that ionic strength and the duration of sonication were the most significant factors impacting fragmentation patterns. A salt concentration of 1 M caused genomic DNA to resist fragmentation and fragment sizes were centred near 500 base pairs and larger. Decreasing the ionic strength to 0.l M NaCl or less resulted in the formation of fragment sizes close to 100 base pairs. Manipulation of these factors allowed for some control of fragmentation patterns. Hyperchromicity experiments, studies considering the influence of various gases, and simulations to determine the probability of destroying a target sequence were also done. Experiments suggested that ultrasound can be used as a rapid method to generate DNA fragments.
590 $a School code: 0779.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
690 $a 0487
710 2 0 $a University of Toronto (Canada). $3 1017674
773 0 $t Masters Abstracts International $g 42-03.
790 1 0 $a Krull, Ulrich, $e advisor
790 $a 0779
791 $a M.Sc.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ84154