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Bead-based immunoassays with electro...

Ronkainen-Matsuno, Niina J.

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Bead-based immunoassays with electrochemical detection.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Bead-based immunoassays with electrochemical detection./
Author:	Ronkainen-Matsuno, Niina J.
Description:	178 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0721.
Contained By:	Dissertation Abstracts International65-02B.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3120889
ISBN:	049668244X

Bead-based immunoassays with electrochemical detection.
Ronkainen-Matsuno, Niina J.

Bead-based immunoassays with electrochemical detection. - 178 p.

Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0721.

Thesis (Ph.D.)--University of Cincinnati, 2003.

Immunoassays with electrochemical detection have gained popularity due to their versatility, low detection limits, relatively easy procedures, and simple instrumentation. Immunoassays have a wide range of applications in clinical chemistry and biodefense as well as agricultural, environmental, and processed food areas. A variety of sensitive amperometric electrochemical detection methods have previously been used with immunoassays. A microelectrode was used here as a detector for bead-based assays in very small sample volumes (down to 10 muL) with low detection limits, fast response times, and fairly good reproducibility. A microelectrode with currents in the pA range and very low charging currents showed great promise as a detector for small volume immunoassays in a biosensor with a microfluidic component. The microelectrode helped overcome the problem of rapid depletion of the electroactive species during detection in small volumes where the macroelectrode would consume the electroactive species faster than the enzymes could produce them. The rapid depletion was seen with macroelectrodes such as the rotating disk electrode.

ISBN: 049668244XSubjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Bead-based immunoassays with electrochemical detection.
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100 1 $a Ronkainen-Matsuno, Niina J. $3 1935739
245 1 0 $a Bead-based immunoassays with electrochemical detection.
300 $a 178 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0721.
500 $a Co-Chairs: H. Brian Halsall; William R. Heineman.
502 $a Thesis (Ph.D.)--University of Cincinnati, 2003.
520 $a Immunoassays with electrochemical detection have gained popularity due to their versatility, low detection limits, relatively easy procedures, and simple instrumentation. Immunoassays have a wide range of applications in clinical chemistry and biodefense as well as agricultural, environmental, and processed food areas. A variety of sensitive amperometric electrochemical detection methods have previously been used with immunoassays. A microelectrode was used here as a detector for bead-based assays in very small sample volumes (down to 10 muL) with low detection limits, fast response times, and fairly good reproducibility. A microelectrode with currents in the pA range and very low charging currents showed great promise as a detector for small volume immunoassays in a biosensor with a microfluidic component. The microelectrode helped overcome the problem of rapid depletion of the electroactive species during detection in small volumes where the macroelectrode would consume the electroactive species faster than the enzymes could produce them. The rapid depletion was seen with macroelectrodes such as the rotating disk electrode.
520 $a Ovalbumin protein, a simulant for biotoxins, used as an antigen in immunoassays was characterized using ion exchange chromatography, thermal denaturation, and circular dichroism. Ovalbumin varied in the degree of phosphorylation of the two serine residues between preparations. A microvolume sandwich immunoassay for ovalbumin with enzyme labels was developed on paramagnetic beads that were easy to manipulate, presented short diffusional distances, and hence faster assay times. A rotating disk electrode at a fixed potential was used as a detector.
520 $a The viability of electrochemical immunoassay as a sensitive technique in a water sample matrix was investigated. Water samples from around the country were characterized with respect to pH and metal content. Sandwich immunoassays for ovalbumin were done in finished water from Cincinnati, OH and from Potomac, MD with minor pretreatment with a chelating agent and a buffering agent.{09}The assay was also done successfully in unfiltered pond water from Cedarville, OH.
590 $a School code: 0045.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Health Sciences, Immunology. $3 1017716
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710 2 0 $a University of Cincinnati. $3 960309
773 0 $t Dissertation Abstracts International $g 65-02B.
790 1 0 $a Halsall, H. Brian, $e advisor
790 1 0 $a Heineman, William R., $e advisor
790 $a 0045
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3120889