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Semi-biosynthesis of DNA nanostructures.

Barhoumi, Aoune.

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Semi-biosynthesis of DNA nanostructures.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Semi-biosynthesis of DNA nanostructures./
作者:	Barhoumi, Aoune.
面頁冊數:	131 p.
附註:	Source: Masters Abstracts International, Volume: 43-02, page: 0536.
Contained By:	Masters Abstracts International43-02.
標題:	Chemistry, Inorganic. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1422485
ISBN:	049603426X

Semi-biosynthesis of DNA nanostructures.
Barhoumi, Aoune.

Semi-biosynthesis of DNA nanostructures. - 131 p.

Source: Masters Abstracts International, Volume: 43-02, page: 0536.

Thesis (M.S.)--Marshall University, 2004.

In this project we demonstrate how we can derive benefit from employing biological techniques, such as Rolling Circle Amplification, Polymerase Chain Reaction, and cloning to address the challenge of emplacing DNA nanoarrays at pre-determined locations on a surface.

ISBN: 049603426XSubjects--Topical Terms:

517253
Chemistry, Inorganic.

Semi-biosynthesis of DNA nanostructures.
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300 $a 131 p.
500 $a Source: Masters Abstracts International, Volume: 43-02, page: 0536.
502 $a Thesis (M.S.)--Marshall University, 2004.
520 $a In this project we demonstrate how we can derive benefit from employing biological techniques, such as Rolling Circle Amplification, Polymerase Chain Reaction, and cloning to address the challenge of emplacing DNA nanoarrays at pre-determined locations on a surface.
520 $a In vitro rolling Circle amplification (RCA) driven by DNA polymerization was first reported by Eric T. Kool and coworkers in 1995. DNA products resulting from RCA are repeating head-to-tail multimeric copies of the DNA template. We report the design and synthesis of both single stranded circular DNA (used as a template) and a multimeric product. Using the RCA technique, long tandem repeats, consisting of multiple copies of a 95 base pair sequence have been produced. We incorporated two specific, unique sequences at each end of these synthesized DNA strands, which can be used as recognition sites for surface hybridization. For the first time, heterogeneity has been introduced into a repetitive system to yield a modular nanostructured macromolecule. This product was further cloned into bacterial host cells. The DNA fragments were extracted and sequenced. The results not only confirm success in these particular experiments, but they also verify the general validity of this technique for generating nano-constructs semi-biosynthetically.
520 $a In order to demonstrate applicability of the RCA product to nanotechnology, we used these strands as scaffolds for gold nanoparticle patterning. (Abstract shortened by UMI.)
590 $a School code: 0817.
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