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Theoretical studies in polynucleotid...

Lubensky, David Koslan.

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Theoretical studies in polynucleotide biophysics.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Theoretical studies in polynucleotide biophysics./
作者:	Lubensky, David Koslan.
面頁冊數:	179 p.
附註:	Source: Dissertation Abstracts International, Volume: 62-10, Section: B, page: 4598.
Contained By:	Dissertation Abstracts International62-10B.
標題:	Physics, Condensed Matter. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3028409
ISBN:	0493408142

Theoretical studies in polynucleotide biophysics.
Lubensky, David Koslan.

Theoretical studies in polynucleotide biophysics. - 179 p.

Source: Dissertation Abstracts International, Volume: 62-10, Section: B, page: 4598.

Thesis (Ph.D.)--Harvard University, 2001.

This thesis investigates the physics of the polynucleotides DNA and RNA, with an emphasis on theory relevant to single molecule experiments. An introductory chapter reviews some facts about these polymers and gives an overview of important experimental techniques. Motivated by attempts to develop new technologies for DNA sequencing and related assays, we turn in the second chapter to the dynamics of polynucleotides threaded through narrow pores. We show that there is a range of polymer lengths in which the system is approximately translationally invariant, and we develop a coarse-grained description of this regime. We also introduce a more microscopic model that provides a physically reasonable scenario in which, as in experiments, the polymer's speed depends sensitively on its chemical composition. Finally, we point out that the experimental distribution of polymer transit times is much broader than expected from simple estimates, and speculate on why this might be.

ISBN: 0493408142Subjects--Topical Terms:

1018743
Physics, Condensed Matter.

Theoretical studies in polynucleotide biophysics.
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245 1 0 $a Theoretical studies in polynucleotide biophysics.
300 $a 179 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-10, Section: B, page: 4598.
500 $a Adviser: David R. Nelson.
502 $a Thesis (Ph.D.)--Harvard University, 2001.
520 $a This thesis investigates the physics of the polynucleotides DNA and RNA, with an emphasis on theory relevant to single molecule experiments. An introductory chapter reviews some facts about these polymers and gives an overview of important experimental techniques. Motivated by attempts to develop new technologies for DNA sequencing and related assays, we turn in the second chapter to the dynamics of polynucleotides threaded through narrow pores. We show that there is a range of polymer lengths in which the system is approximately translationally invariant, and we develop a coarse-grained description of this regime. We also introduce a more microscopic model that provides a physically reasonable scenario in which, as in experiments, the polymer's speed depends sensitively on its chemical composition. Finally, we point out that the experimental distribution of polymer transit times is much broader than expected from simple estimates, and speculate on why this might be.
520 $a The third chapter gives a brief account, focusing on behavior averaged over many random sequences, of work on the mechanical pulling apart of the two strands of double-stranded DNA (dsDNA). When the pulling force is increased to a critical value (typically of order 10 pN), an "unzipping" transition occurs. For random DNA sequences with short-ranged correlations, we obtain exact results for the number of monomers liberated, including the critical behavior at the transition.
520 $a The final chapter expands upon these results on the unzipping transition, providing more details of our disorder-averaged calculations and tackling the more experimentally accessible problem of the unzipping of a single dsDNA molecule. As the applied force approaches the critical value, a given dsDNA unravels in a series of discrete, sequence dependent steps that allow it to reach successively deeper energy minima. Plots of extension versus force thus take the striking form of a series of plateaus separated by sharp jumps. Similar qualitative features should reappear in experiments on folded RNAs. Above the transition, the dynamics of the unzipping fork is related to that of a particle diffusing in a random force field; anomalous, disorder-dominated behavior is expected until the applied force exceeds the critical force by roughly 5 pN.
590 $a School code: 0084.
650 4 $a Physics, Condensed Matter. $3 1018743
650 4 $a Biophysics, General. $3 1019105
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773 0 $t Dissertation Abstracts International $g 62-10B.
790 1 0 $a Nelson, David R., $e advisor
790 $a 0084
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3028409