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The extent and rate of antigen-antib...

Swift, David Glenn.

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The extent and rate of antigen-antibody-mediated adhesion of rat basophilic leukemia cells to surfaces in a linear shear field.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The extent and rate of antigen-antibody-mediated adhesion of rat basophilic leukemia cells to surfaces in a linear shear field./
作者:	Swift, David Glenn.
面頁冊數:	340 p.
附註:	Source: Dissertation Abstracts International, Volume: 59-11, Section: B, page: 5747.
Contained By:	Dissertation Abstracts International59-11B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9913523
ISBN:	0599121300

The extent and rate of antigen-antibody-mediated adhesion of rat basophilic leukemia cells to surfaces in a linear shear field.
Swift, David Glenn.

The extent and rate of antigen-antibody-mediated adhesion of rat basophilic leukemia cells to surfaces in a linear shear field. - 340 p.

Source: Dissertation Abstracts International, Volume: 59-11, Section: B, page: 5747.

Thesis (Ph.D.)--University of Pennsylvania, 1998.

Many physiological and biotechnological processes rely on the receptor-mediated adhesion of cells to surfaces under flow. The ability of adhesion molecules to support cell adhesion under flow, and the dynamic mode of adhesion, is determined by the physiochemical parameters of the system, including binding kinetics, their response to stress, and their site densities on the cell and surface. In this study, we examine the parameter dependence of adhesion of antibody-functionalized rat basophilic leukemia cells to antigen-derivatized polyacrylamide gels in a linear shear field. A library approach is used, in which different antibody-antigen pairs of known binding kinetics and affinity are examined for their ability to support cell adhesion and rolling at a range of fluid forces. Four antibody and antigen pairs are used, the two murine monoclonal IgE clones SPE-7 and H1 26.82, both raised against nitrosylphenol haptens, and the two nitrosylphenol haptens, trinitrophenyl (TNP) and 2,4-dinitrophenyl (DNP). All but the pair with the slowest rate of binding, SPE-7/TNP, support adhesion under flow. The HI 26.82/TNP pair supports both firm and transient adhesion and the remaining pairs support only firm adhesion. The extent and rate of adhesion is greatest for the antibody-antigen pairs with the fastest kinetic rates of association. The wall shear rate and molecular site densities are strong modulators of adhesion. The extent of adhesion decreases with increasing shear rate, while the first order adhesion rate constant increases, decreases, or remains constant with increasing shear rate, depending on the antibody and antigen site densities. In this study we also illustrate a novel method for cell separation based on the rate of adhesion of cells to a surface. Cells differing in ether antibody-antigen binding rate or receptor density are separated using this technique. Rate-based cell selection techniques have the potential to permit fine separations in a wide variety of applications in medicine and biotechnology.

ISBN: 0599121300Subjects--Topical Terms:

1019105
Biophysics, General.

The extent and rate of antigen-antibody-mediated adhesion of rat basophilic leukemia cells to surfaces in a linear shear field.
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