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The regulation and expression of ear...

Fraley, Cresson Dodge.

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The regulation and expression of early starvation-induced genes in Escherichia coli.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The regulation and expression of early starvation-induced genes in Escherichia coli./
作者:	Fraley, Cresson Dodge.
面頁冊數:	104 p.
附註:	Source: Dissertation Abstracts International, Volume: 59-10, Section: B, page: 5517.
Contained By:	Dissertation Abstracts International59-10B.
標題:	Engineering, Environmental. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9908756
ISBN:	0599069120

The regulation and expression of early starvation-induced genes in Escherichia coli.
Fraley, Cresson Dodge.

The regulation and expression of early starvation-induced genes in Escherichia coli. - 104 p.

Source: Dissertation Abstracts International, Volume: 59-10, Section: B, page: 5517.

Thesis (Ph.D.)--Stanford University, 1998.

Upon entry into stationary phase due to starvation, Escherichia coli cells undergo rapid changes in gene expression. The alternate sigma factor RpoS (

ISBN: 0599069120Subjects--Topical Terms:

783782
Engineering, Environmental.

The regulation and expression of early starvation-induced genes in Escherichia coli.
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245 1 4 $a The regulation and expression of early starvation-induced genes in Escherichia coli.
300 $a 104 p.
500 $a Source: Dissertation Abstracts International, Volume: 59-10, Section: B, page: 5517.
500 $a Adviser: A. C. Matin.
502 $a Thesis (Ph.D.)--Stanford University, 1998.
520 $a Upon entry into stationary phase due to starvation, Escherichia coli cells undergo rapid changes in gene expression. The alternate sigma factor RpoS ( $\ sigma\sp{\rm s} $, KatF) is centrally involved in this process, regulating the expression of more than thirty genes required for stationary phase survival. To understand rpoS regulation, a series of transcriptional and translational $\ sp\prime $l acZ fusions were made inside the open reading frame (ORF). In minimal medium with limited glucose as sole carbon source, $\ beta $- galactosidase expression increased about two-fold upon starvation with the longest transcriptional ORF fusion. The equivalent translational fusion exhibited a greater than eight-fold induction. Similar results were obtained in complex medium regardless of the ORF fragment length. Thus, although some regulation of rpoS expression under carbon starvation conditions occurs at the transcriptional level, the principal regulation is posttranscriptional.
520 $a $c stC $ is a starvation-induced gene which is regulated in part by RpoS. Transcriptional $\ sp\prime $l acZ fusions demonstrated that, like rpoS, it is induced under nitrogen as well as carbon starvation conditions. However, during nitrogen starvation, expression occurs from a putative $\ sigma\sp{54} $ promoter site. Under this condition, RpoS may inhibit transcription due to the overlap of its binding site with that of $\ sigma\sp{54} $. Under carbon starvation, transcription occurs from both promoters although $\ sigma\sp{54} $ is apparently dispensable. cstC mutants are impaired in utilizing amino acids as sole nitrogen source and in starvation survival relative to the wild type. The cstC ORF is highly homologous to the arginine synthetic enzyme ArgD and appears to be its catabolic counterpart.
520 $a Starvation-induced gene expression is of fundamental biological interest and is also potentially useful for biotechnology applications such as the in situ bioremediation of contaminated environments. To minimize the biomass required to achieve a given level of bioremediation activity, E. coli starvation-induced promoters were used to control the synthesis of toluene-4-monooxygenase. This enzyme degrades the common toxic environmental contaminants trichloroethylene (TCE) and aromatic hydrocarbons such as phenol. Rapid conversions of TCE and phenol occurred in starved batch or very slow growth chemostat cultures. In these experiments, normalized biomass production and glucose utilization were reduced by 60 to greater than 90% relative to conventional systems. These results demonstrate that in principle, starvation-induced gene expression systems have significant potential for biotechnological applications.
590 $a School code: 0212.
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
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710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 59-10B.
790 1 0 $a Matin, A. C., $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 1998
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9908756