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Cyclins, CDKs, and cyclin-specific t...

Levine, Kristi.

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Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae./
作者:	Levine, Kristi.
面頁冊數:	238 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-07, Section: B, page: 3089.
Contained By:	Dissertation Abstracts International60-07B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9936931
ISBN:	0599382341

Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae.
Levine, Kristi.

Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae. - 238 p.

Source: Dissertation Abstracts International, Volume: 60-07, Section: B, page: 3089.

Thesis (Ph.D.)--The Rockefeller University, 1999.

Progression through the cell cycle is driven by the activity of the cyclin-dependent kinases, or CDKs. CDK activity is dependent upon their physical association with cyclins. The cell cycle of the budding yeast Saccharomyces cerevisiae is controlled primarily by the CDK known as Cdc28, activated by nine yeast cyclins, Cln1-3 and Clb1-6. The different cyclin-Cdc28 complexes are specialized to promote distinct cell cycle events. In part, their functional specificities reflect quantitative differences, resulting from their differential expression, degradation, and inhibition. In addition, their functional specificities may reflect qualitative differences, due to cyclin-specific targeting of the complexes to particular substrates. The studies described in this thesis have been designed to investigate the potential presence, basis, and significance of cyclin-specific targeting in budding yeast.

ISBN: 0599382341Subjects--Topical Terms:

1017730
Biology, Genetics.

Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae.
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245 1 0 $a Cyclins, CDKs, and cyclin-specific targeting in Saccharomyces cerevisiae.
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500 $a Source: Dissertation Abstracts International, Volume: 60-07, Section: B, page: 3089.
500 $a Adviser: Frederick R. Cross.
502 $a Thesis (Ph.D.)--The Rockefeller University, 1999.
520 $a Progression through the cell cycle is driven by the activity of the cyclin-dependent kinases, or CDKs. CDK activity is dependent upon their physical association with cyclins. The cell cycle of the budding yeast Saccharomyces cerevisiae is controlled primarily by the CDK known as Cdc28, activated by nine yeast cyclins, Cln1-3 and Clb1-6. The different cyclin-Cdc28 complexes are specialized to promote distinct cell cycle events. In part, their functional specificities reflect quantitative differences, resulting from their differential expression, degradation, and inhibition. In addition, their functional specificities may reflect qualitative differences, due to cyclin-specific targeting of the complexes to particular substrates. The studies described in this thesis have been designed to investigate the potential presence, basis, and significance of cyclin-specific targeting in budding yeast.
520 $a The in vivo functional specificities of Cln1 and Cln2 are distinct from those of Cln3. To ask if qualitative differences between Cln-Cdc28 complexes contribute to their functional specificities we compared CLN2, CLN3 , and mutant CLN2 genes in a range of assays that genetically differentiate between CLN2 and CLN3. As a simple quantitative model cannot explain the results, it is likely that Cln2-Cdc28 and Cln3-Cdc28 are qualitatively different, and are targeted to phosphorylate distinct substrates in vivo.
520 $a It is possible that CDKs contain regions or residues involved in cyclin-specific interactions. These interactions may allow for efficient cyclin-CDK binding and activation, and/or may contribute to the functional specificities of cyclin-CDK complexes via modulation of the substrate binding site. In an attempt to identify cyclin-specific interaction domains of Cdc28, we screened for CDC28 alleles with cyclin-specific functions. The screens produced CDC28 mutants with diverse biochemical defects in cyclin binding and activation.
520 $a The generation of a cyclin-independent activated CDK would allow the independent evaluation of the activating and targeting roles played by cyclins. Through a series of genetic screens, we identified alleles of CDC28 with CLN-independent biological functions. Biochemical analysis of one of the CLN-independent alleles revealed a low level of cyclin-independent biochemical activity. Our findings may suggest that at least some CLN-specific functional roles are independent of cyclin-specific targeting, yet are consistent with the suggestion of qualitative differences between Cln-Cdc28 complexes.
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