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Suppressors of dominant negative SEC...

Shannon, Jeffrey Francis.

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Suppressors of dominant negative SEC4 alleles.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Suppressors of dominant negative SEC4 alleles./
作者:	Shannon, Jeffrey Francis.
面頁冊數:	110 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1956.
Contained By:	Dissertation Abstracts International60-05B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9931059
ISBN:	0599311428

Suppressors of dominant negative SEC4 alleles.
Shannon, Jeffrey Francis.

Suppressors of dominant negative SEC4 alleles. - 110 p.

Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1956.

Thesis (Ph.D.)--Yale University, 1999.

Vesicular transport from the Golgi to the plasma membrane in Saccharomyces cerevisiae is dependent on ten sec genes. Sec4p is a Ras superfamily GTPase that has an essential role in this transport step and is a thought to act as a molecular switch. Activation of the switch occurs when GDP is exchanged for GTP bound to Sec4p. This activation is catalyzed by a diverse class of proteins known as guanine nucleotide exchange factors (GEFs).

ISBN: 0599311428Subjects--Topical Terms:

1017686
Biology, Cell.

Suppressors of dominant negative SEC4 alleles.
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100 1 $a Shannon, Jeffrey Francis. $3 1927874
245 1 0 $a Suppressors of dominant negative SEC4 alleles.
300 $a 110 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 1956.
500 $a Director: Peter J. Novick.
502 $a Thesis (Ph.D.)--Yale University, 1999.
520 $a Vesicular transport from the Golgi to the plasma membrane in Saccharomyces cerevisiae is dependent on ten sec genes. Sec4p is a Ras superfamily GTPase that has an essential role in this transport step and is a thought to act as a molecular switch. Activation of the switch occurs when GDP is exchanged for GTP bound to Sec4p. This activation is catalyzed by a diverse class of proteins known as guanine nucleotide exchange factors (GEFs).
520 $a To identify putative GEFs we over-expressed known secretory genes and screened a high copy genomic library for suppressors of two dominant negative alleles of SEC4, SEC4N34 and SEC4 I133. GEFs have been shown to form complexes with Ras-like GTPases in their nucleotide free state and the dominant negative proteins mimic this state and form non-productive complexes with GEFs.
520 $a Isolation of suppressors of SEC4N34 and SEC4I133 revealed that these two mutants may have common and distinct sites of action. Over-expression of Sec2p shows much stronger suppression of the SEC4N34 allele. Sec2p has recently been shown to be a potent exchange factor for Sec4p and will bind both Sec4N34p and Sec4I133p by two hybrid assay (Walch-Solimena et al. 1997). Truncations of the Sec2 protein decrease its localization to sites of polarized growth and abolish suppression of SEC4I133 (Elkind et al 1997).
520 $a Sec4N34p and Sec4I133p may share Sec2p as a target and Sec4I133p may have an additional target at or on the way to the plasma membrane. Specific suppressors of each allele seem to support this hypothesis. YPT31, a Rab protein implicated in the exit of vesicles from the Golgi, shows specific suppression of SEC4N34. Specific suppressors of SEC4 I133 Sso2p and Smy1p are localized to the plasma membrane and the bud tip respectively.
520 $a Immunofluorescence of the dominant negative proteins shows a diffuse punctate appearance, suggesting localization to accumulated vesicles. This punctate pattern of Sec4I133p is frequently found in the bud tip while Sec4N34p is not. Localization of Sec2-GFP and Sec4-GFP in these backgrounds also supports the hypothesis that Sec4N34p and Sec4I133p have distal and proximal targets with respect to the plasma membrane.
590 $a School code: 0265.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0379
690 $a 0369
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710 2 0 $a Yale University. $3 515640
773 0 $t Dissertation Abstracts International $g 60-05B.
790 1 0 $a Novick, Peter J., $e advisor
790 $a 0265
791 $a Ph.D.
792 $a 1999
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