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Regulation of the microtubule cytosk...

Feierbach, Becket Lyn.

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Regulation of the microtubule cytoskeleton in yeast.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Regulation of the microtubule cytoskeleton in yeast./
作者:	Feierbach, Becket Lyn.
面頁冊數:	203 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-04, Section: B, page: 1388.
Contained By:	Dissertation Abstracts International60-04B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9924423
ISBN:	059923959X

Regulation of the microtubule cytoskeleton in yeast.
Feierbach, Becket Lyn.

Regulation of the microtubule cytoskeleton in yeast. - 203 p.

Source: Dissertation Abstracts International, Volume: 60-04, Section: B, page: 1388.

Thesis (Ph.D.)--Stanford University, 1999.

Microtubules are cytoskeletal elements found in all eukaryotes. Microtubules are essential to numerous cellular processes and are composed of heterodimers of alpha- and beta-tubulin. In the budding yeast, Saccharomyces cerevisae , the microtubule cytoskeleton is simple and yet displays many of the properties found in higher eukaryotes, such as dynamic behavior. Due to the ease of genetics in yeast and the availibility of a complete genome sequence, yeast has been used extensively in the study of the microtubule cytoskeleton and its associated proteins. This dissertation presents a genetic and biochemical analysis of four yeast genes that are involved with the function of the microtubule cytoskelton. None of these genes are essential for viability, but strains lacking any of these genes shows defects in the microtubule cytoskeleton. Chapter I focuses on the gene Alpha-tubulin Formation 1 (ALF1) whose protein product appears to be an alpha-tubulin monomer-binding protein and may act in regulating heterodimer formation. In addition, I have mapped the region of alpha-tubulin to which the Alf I protein binds, and found it to be a different region than that to which beta-tubulin binds. Chapter 2 focuses on the Chromosome INstability genes, CIN1, 2 and 4. Cin1 protein interacts with beta-tubulin and may participate in the formation of the tubulin heterodimer as well. The CIN2 and CIN4 gene products genetically and physically interact but their precise function remains unknown. Chapter 3 focuses on the peripherally related topic of yeast cell polarity. I have found that the MPM-2 antibody reacts with an epitope at the constriction point between mother and bud. This constriction, called the neck, is important for regulation of cell polarity and cytokinesis. The MPM-2 antibody recognizes phosphorylated epitopes in mitosis and evidence suggests that it recognizes the bud polarity protein, Bud3, at the bud neck. In addition, the mitotic kinase CDC5, is required for the formation of the epitope and may be directly phosphorylating BUD3 in a cell-cycle dependent manner.

ISBN: 059923959XSubjects--Topical Terms:

1017686
Biology, Cell.

Regulation of the microtubule cytoskeleton in yeast.
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