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Influence of nitric oxide release on...

Nablo, Brian Joseph.

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Influence of nitric oxide release on bacterial adhesion and tissue-implant viability.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Influence of nitric oxide release on bacterial adhesion and tissue-implant viability./
作者:	Nablo, Brian Joseph.
面頁冊數:	169 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-07, Section: B, page: 3438.
Contained By:	Dissertation Abstracts International65-07B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3140375
ISBN:	0496875165

Influence of nitric oxide release on bacterial adhesion and tissue-implant viability.
Nablo, Brian Joseph.

Influence of nitric oxide release on bacterial adhesion and tissue-implant viability. - 169 p.

Source: Dissertation Abstracts International, Volume: 65-07, Section: B, page: 3438.

Thesis (Ph.D.)--The University of North Carolina at Chapel Hill, 2004.

The biological significance and benefit of nitric oxide (NO) releasing coatings for biomaterials is investigated by examining the resistance to bacterial adhesion and the implant host response. For this, numerous sol-gel films have been developed that act as a source of NO. Organosilanes containing two or three amines (aminosilanes) reacted with isobutyltrimethoxysilane form stable, translucent sol-gel coatings capable of releasing nitric oxide (NO) after exposure to a NO atmosphere. The NO release can be tuned by varying the amount and type of aminosilane up to 19.0 pmol s-1 cm -2. Pseudomonas aeruginosa adhesion under static and dynamic conditions is evaluated at multiple sol-gel formulations. A decrease in cellular adhesion is observed with an increase in NO release, with up to a 95% decrease in cell coverage. The benefit of NO-releasing sol-gels as orthopedic coatings is examined with bacterial adhesion at medical-grade stainless steel. The in vitro adhesion of P. aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis under ambient and physiological temperatures decreases up to 80% in the presence of a NO. The relationship between NO flux and bacterial adhesion is determined by coating NO-releasing sol-gels with a polymer film. The polymer provides a control surface for monitoring bacterial adhesion, since a change in sol-gel composition is required to modify the NO release. A 5--35% decrease in NO flux is observed after coating sol-gel films with 10--30 mum of poly(vinyl chloride). A relationship between the resistance toward P. aeruginosa adhesion and NO release is discovered over surface fluxes of 0--30 pmol s-1 cm-2. The host response to NO-releasing sol-gel is examined in vitro with L929 mouse fibroblasts to assess cytotoxicity and subcutaneous implantation in rats to examine the tissue response. Low levels of NO release from sol-gels produced a highly cytotoxic effect on fibroblasts in vitro. Contrary, the in vivo implantation results suggest that NO-releasing sol-gels coatings reduce dense collagen deposition, are not necrotic, and induce angiogenesis. The inhibition of bacterial adhesion and stimulation of subcutaneous tissue growth demonstrate the potential advantages of integrating sol-gel based NO release into the designs of current implantable medical devices and bio sensors.

ISBN: 0496875165Subjects--Topical Terms:

586156
Chemistry, Analytical.

Influence of nitric oxide release on bacterial adhesion and tissue-implant viability.
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