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Dynamics of iron active sites in hem...

Rai, Brajesh Kumar.

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Dynamics of iron active sites in heme proteins and model compounds.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Dynamics of iron active sites in heme proteins and model compounds./
作者:	Rai, Brajesh Kumar.
面頁冊數:	171 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4821.
Contained By:	Dissertation Abstracts International64-10B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3108402
ISBN:	0496559470

Dynamics of iron active sites in heme proteins and model compounds.
Rai, Brajesh Kumar.

Dynamics of iron active sites in heme proteins and model compounds. - 171 p.

Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4821.

Thesis (Ph.D.)--Purdue University, 2003.

Vibrational dynamics of iron active sites in heme proteins and model compounds have been studied by refining theoretical calculations to nuclear resonance vibrational spectroscopy (NRVS) data. The NRVS measurements give quantitative information on the frequencies and iron amplitudes of all modes having significant Fe vibrational motion. Normal mode analysis is used to identify and characterize the iron vibrational modes of the heme models, (Nitrosyl)iron (II)tetraphenylporphyrin, [Fe(TPP)(NO)], (2-methylimidazole)(tetraphenylporphinato)iron (II). [Fe(TPP)(2-McHIm)], and six-coordinate [Fe(TPP)(1-MeIm)(CO)]. Modes having large overlaps with in-plane nu42, nu50, and nu53 vibrations of the porphyrin core are identified, as well as several modes with large stretch and bend components of axial ligands. The out-of-plane Fe modes with significant doming of the porphyrin core are found near 75 cm-1, but the largest Fe doming motion arises from the coupling of the phenyls near 30 cm-1. Significantly, the normal mode analysis of the model compounds, in conjunction with NRVS data, provides a good set of refined force constants, which is used to study the effects of protein environment on the active sites of heme proteins.

ISBN: 0496559470Subjects--Topical Terms:

1019105
Biophysics, General.

Dynamics of iron active sites in heme proteins and model compounds.
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245 1 0 $a Dynamics of iron active sites in heme proteins and model compounds.
300 $a 171 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4821.
500 $a Major Professor: Earl W. Prohofsky.
502 $a Thesis (Ph.D.)--Purdue University, 2003.
520 $a Vibrational dynamics of iron active sites in heme proteins and model compounds have been studied by refining theoretical calculations to nuclear resonance vibrational spectroscopy (NRVS) data. The NRVS measurements give quantitative information on the frequencies and iron amplitudes of all modes having significant Fe vibrational motion. Normal mode analysis is used to identify and characterize the iron vibrational modes of the heme models, (Nitrosyl)iron (II)tetraphenylporphyrin, [Fe(TPP)(NO)], (2-methylimidazole)(tetraphenylporphinato)iron (II). [Fe(TPP)(2-McHIm)], and six-coordinate [Fe(TPP)(1-MeIm)(CO)]. Modes having large overlaps with in-plane nu42, nu50, and nu53 vibrations of the porphyrin core are identified, as well as several modes with large stretch and bend components of axial ligands. The out-of-plane Fe modes with significant doming of the porphyrin core are found near 75 cm-1, but the largest Fe doming motion arises from the coupling of the phenyls near 30 cm-1. Significantly, the normal mode analysis of the model compounds, in conjunction with NRVS data, provides a good set of refined force constants, which is used to study the effects of protein environment on the active sites of heme proteins.
520 $a The vibrational spectra of iron active sites in myoglobin and in alpha and beta subunits of hemoglobin are calculated using a Green-function approach, and the results are compared to NRVS data. The method allows us to focus on the heme active site, and gives insights into the effects of globin coupling on the functionally important heme iron modes. The most prominent vibrational feature of the iron VDOS spectra (i.e. the 250 cm-1 band) of myoglobin, as well as that from the alpha and beta subunits of hemoglobin, have been assigned to the localized nu53 modes of heme. While the high frequency modes of both rnyoglobin and hemoglobin couple weakly to the globin, the low frequency features in the vibrational spectra are significantly altered by the protein environment. The globin-driven translations of the entire heme contribute to the 25 cm-1 feature of the VDOS spectra of myoglobin and hemoglobin. The coupling of globin to the heme produces numerous weak and delocalized Fe out-of-plane modes at the intermediate frequencies (60--140 cm-1) that overlap with the doming coordinate of the porphyrin core. We find that the iron out-of-plane motion at different active sites in hemoglobin is most strongly correlated for a couple modes at &sim;93 cm-1, suggesting that the biologically significant cooperative motions of hemoglobin occur at intermediate frequencies.
590 $a School code: 0183.
650 4 $a Biophysics, General. $3 1019105
690 $a 0786
710 2 0 $a Purdue University. $3 1017663
773 0 $t Dissertation Abstracts International $g 64-10B.
790 1 0 $a Prohofsky, Earl W., $e advisor
790 $a 0183
791 $a Ph.D.
792 $a 2003
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